摘要:AP Buffer:100 mM Tris-HCl, pH 9.5 100 mM NaCl 10 mM MgCl2 PTM:PBS 0.1% Tween-20 2 mM MgCl2Since this is generally done in conjunction with lacZ staining, embryo/tissue is usually fixed as per lacZ sta[阅读全文:]
摘要:INTRODUCTION After chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is precipitated at a locus of interest. Real-time PCR amplification is oft[阅读全文:]
摘要:Use 4ml polycarbonate snap-cap tubes for all steps. Rocking of tubes should be performed at all times. All steps are carried out at room temperature unless otherwise stated. Day 0 1. Dissect embryos i[阅读全文:]
摘要:染色质免疫沉淀法（Chromatin immunoprecitation，ChIP）是研究体内DNA与蛋白质相互作用的重要工具。它可以灵敏地检测目标蛋白与特异DNA片段的结合情况，还可以用来研究组蛋白与基因表达的关系。核小体组蛋白可以发生多种[阅读全文:]
摘要:Many methods have been developed to identify genomic targets of DNA-binding factors. Here we outline and discuss our adaptation of the chromatin immunoprecipitation (ChIP) assay to a high-throughput m[阅读全文:]
摘要:Formaldehyde cross-linking and chromatin immunoprecipitation assays of tissue culture cells are performed as described by Boyd and Farnham (MCB 29:8389-8399) and Y. Shang et. al. (Cell 103:843-852) wi[阅读全文:]
摘要:Human chromosome dimensions Chromosome # Relative length Centromere index 1 9.08 48.0 2 8.45 38.1 3 7.06 45.9 4 6.55 27.6 5 6.13 27.4 6 5.84 37.7 7 5.28 37.3 X 5.80 36.9 8 4.96 35.9 9 4.83 33.3 10 4.6[阅读全文:]
摘要:试剂Earles Balanced Salt Solution (BSS) earlesFetal Bovine SerumGiemsa stain, original azure blend GiemsaHarleco 620G/75 harleco 620g/75Gurrs buffer tablets, pH 6.8 gurrsBiomedical Specialties, Santa [阅读全文:]
摘要:Reagents dH 2OEthanol, absolute (70%, 90%, and 100%)Formamide, deionizedAmbion, Cat. No. 9342HCL, 1NRubber CementSSC, 20X Preparation70% Formamide/2X SSC20X SSC 10 mldH 2O 20 mldeionized formamide 70 [阅读全文:]