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"细胞生物学技术" 分类下的词条该分类下有1199个词条创建该分类下的词条

词条创建者:admin创建时间:12-08 00:48
标签: 细胞培养,常见问题,解答

摘要:1.如何选用培养基?培养某一类型细胞没有固定的培养条件。在MEM中培养的细胞,很可能在DMEM或M199中同样很容易生长。总之,首选MEM做粘附细胞培养、RPMI-1640做悬浮细胞培养,各种目的无血清培养的首选是AIM V培养基(SFM)。 [阅读全文:]

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Subculture of Suspension Cell Lines
词条创建者:admin创建时间:12-08 00:48
标签: subculture suspension cell 传代培养 悬浮细胞

摘要:1.Basic Techniques - The 'Do's and Don'ts' of Cell CultureGiven below are a few of the essential "do's and don'ts" of cell culture. Some of these are mandatory e.g. use of personal protective equipmen[阅读全文:]

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Serum Thawing & Heat Inactivation
词条创建者:admin创建时间:12-08 00:48
标签: Serum Thawing 热灭活

摘要:How to thaw serum:Serum that is stored at -10º C to -40º C is stable for extended periods of time. It is neither necessary or desirable to store serum at -70º C, as it does not prolong [阅读全文:]

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Direct tissue explant method for derivation and study of extravillous trophoblas
词条创建者:admin创建时间:12-08 00:48
标签: 组织分离法 滋养层 tissue explant method trophoblas

摘要:From early post-implantation stages to approximately 18 weeks' gestation, cytotrophoblast at the tips of anchoring villi at the placental periphery proliferate to form columns. From the distal edges o[阅读全文:]

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Staining protocols for placental explant cultures
词条创建者:admin创建时间:12-08 00:48
标签: staining placental explant cultures 胎盘 移植培养 染色法

摘要:Whole mount staining.The protocol given uses a single primary antibody with nuclear counterstaining, but it can be extended to double antibody staining. It requires an inverted microscope with fluores[阅读全文:]

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Establishment of a Primary Culture
词条创建者:admin创建时间:12-08 00:48
标签: primary culture 原代培养

摘要:MaterialsChick embryo (approximately 8 days old)70% (v/v) ethanol for swabbingSterile scissors, forceps and probesSterile petri platesPhosphate buffered saline (PBS)Trypsin, cold sterilized in a 125 m[阅读全文:]

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Flow Cytometric Analysis Cell Cycle
词条创建者:admin创建时间:12-08 00:48
标签: Flow Cytometric Analysis Cell Cycle 流式细胞 周期

摘要:Fixation1) Collect 2 X 106 cells.2) Pellet cells by spinning at 1,000 rpm, 4°C for 5 minutes.3) Resuspend cell pellet in 1 ml of cold PBS.4) Fix cells by adding 4 ml of -20°C absolute ethanol.[阅读全文:]

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词条创建者:admin创建时间:12-08 00:48
标签: Paraformaldehyde Fixation Cells 甲醛 固定 细胞

摘要:Paraformaldehyde Fixation of Cells BackgroundThis fixation method is good for cells labelled by fluorochrome-conjugated antibodies to membrane antigens. It will stabilize the light scatter and labelli[阅读全文:]

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FIXATION and DNA Staining for Cell Cycle Analysis
词条创建者:admin创建时间:12-08 00:48
标签: FIXATION DNA Staining Cell Cycle DNA染色 固定 细胞周期

摘要:BackgroundThis method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to enter the cells. Propidium Iodide (PI) is a DNA-bindin[阅读全文:]

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词条创建者:admin创建时间:12-08 00:48
标签: Passaging cells

摘要:Passaging cellsPour out media from flasks. Wash with Hanks. 5 ml per flask. Tilt around then dump. Add 4 ml of Trypsin / EDTA to each flask. Tip, then bang. Add 20% FBS NCTC media and tilt. 4 ml per f[阅读全文:]

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细胞生物学技术