摘要:1.如何选用培养基?培养某一类型细胞没有固定的培养条件。在MEM中培养的细胞,很可能在DMEM或M199中同样很容易生长。总之,首选MEM做粘附细胞培养、RPMI-1640做悬浮细胞培养,各种目的无血清培养的首选是AIM V培养基(SFM)。 [阅读全文:]
摘要:1.Basic Techniques - The 'Do's and Don'ts' of Cell CultureGiven below are a few of the essential "do's and don'ts" of cell culture. Some of these are mandatory e.g. use of personal protective equipmen[阅读全文:]
摘要:How to thaw serum:Serum that is stored at -10º C to -40º C is stable for extended periods of time. It is neither necessary or desirable to store serum at -70º C, as it does not prolong [阅读全文:]
摘要:From early post-implantation stages to approximately 18 weeks' gestation, cytotrophoblast at the tips of anchoring villi at the placental periphery proliferate to form columns. From the distal edges o[阅读全文:]
摘要:Whole mount staining.The protocol given uses a single primary antibody with nuclear counterstaining, but it can be extended to double antibody staining. It requires an inverted microscope with fluores[阅读全文:]
摘要:MaterialsChick embryo (approximately 8 days old)70% (v/v) ethanol for swabbingSterile scissors, forceps and probesSterile petri platesPhosphate buffered saline (PBS)Trypsin, cold sterilized in a 125 m[阅读全文:]
摘要:Fixation1) Collect 2 X 106 cells.2) Pellet cells by spinning at 1,000 rpm, 4°C for 5 minutes.3) Resuspend cell pellet in 1 ml of cold PBS.4) Fix cells by adding 4 ml of -20°C absolute ethanol.[阅读全文:]
摘要:Paraformaldehyde Fixation of Cells BackgroundThis fixation method is good for cells labelled by fluorochrome-conjugated antibodies to membrane antigens. It will stabilize the light scatter and labelli[阅读全文:]
摘要:BackgroundThis method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to enter the cells. Propidium Iodide (PI) is a DNA-bindin[阅读全文:]