摘要:immunofluorescence labeling; that is, the antibodies have the fluorescent dye attached. Direct labeling is simpler and quicker than indirect labeling. We strongly recommend direct labeling if you're p[阅读全文:]
摘要:BrdU Incorporation ProtocolDay 1Label cells with media containing100 μM BrdU (6 μL/mL of 5 mg/mL stock): Diploid Fibroblasts, 4-6 hours ES cells, 30 minutes 3T3 cells, 2.5 hours Trypsinize cells[阅读全文:]
摘要:Protocol I: Triton X-100 Lysis Buffer In 96 flat-wells plate, incubate 4x10 6 target cells (40 wells of 105 per well) with desired concentration of effectors (105 target cells per well). After incubat[阅读全文:]
摘要:Materials from a workshop held March, 1997 in Modena, Italy. All materials reproduced with permission.Introduction A. CossarizzaWorkshop SponsorsMethods in analysis of apoptosis and cell necrosis. Z. [阅读全文:]
摘要:以下是根据NIH老年病研究所提供的英文的R1胚胎干细胞培养protocol整理而成。根据经验作部分修改。1、一般培养：保持胚胎干细胞处于未分化状态培养基细胞复苏冻存细胞明胶包被细胞传代2 、体外分化培养基包被有多聚鸟氨酸/[阅读全文:]
摘要:Protocol for Paraffin Sections: Dewax paraffin sections: Incubate slides, 55°C, 30 min.Xylenes, 2 times, 2 min. each100% EtOH, 2 times, 2 min. each95% EtOH, 2 times, 2 min. each80% EtOH, 2 min.75%[阅读全文:]
摘要:Day 0One frozen vial of Murine Embryonic Fibroblasts (MEFs) is thawed quickly in a 37oC water bath. When the last bit of ice is melted, spray the vial with 70% ethanol and transfer the contents of the[阅读全文:]
摘要:1.Ice on tray.2. Dissection tools: 1 pair of large scissors; 2 pairs of fine scissors; 1 pair of coarse forceps; 2 pairs of fine forceps; soak in 95% EtOH.3. 70% EtOH in a spray bottle.4. 2 sets of 10[阅读全文:]