摘要:The technique described here is a slight modification (March 1997) of methods presented in: Nagy A., J. Rossant. 1993. Production of completely ES cell-derived fetuses. In : Gene Targeting: A Practica[阅读全文:]
摘要:一、原代细胞培养 (一)原理：细胞培养(Cell culture)是模拟机体内生理条件，将细胞从机体中取出，在人工条件下使其生存、生长、繁殖和传代，进行细胞生命过程、细胞癌变、细胞工程等问题的研究。近年来，广泛地应用于分子生物学[阅读全文:]
摘要:这里是一份GIBCO的低糖DMEM粉剂配制说明（普通高糖的DMEM培养基配法是一样的）：TO PREPARE 1*LIQUID 1.、Measrue out 5% less distilled water than desired total volume of medium using a mixing container that is [阅读全文:]
摘要:Three weeks prior to embryonic fibroblast isolation, a PGK-neo male mouse is mated to a heterozygote female. 13.5 days to 14.5 days after the plug is observed, sacrifice the pregnant female either by [阅读全文:]
摘要:1.Treat 10 cm tissue culture plates with 0.1% gelatin for at least two hours before use. 2.Sacrifice the pregnant female mouse (day 13 or 14 p.c.) by cervical dislocation. Dissect out the uterine horn[阅读全文:]
摘要:Day -1Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells.Day 1Trypsinized the cells as for normal passaging until the colonies lift off. Try to [阅读全文:]
摘要:Day -1? Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells.Day 1? Trypsinized the cells as for normal passaging until the colonies lift off. Try[阅读全文:]
摘要:Day -1Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells.Day 1Trypsinized the cells as for normal passaging until the colonies lift off. Try to [阅读全文:]
摘要:支原体菌株来源：M.Arginini ATCC23838 精氨酸支原体M.FermentaneATCC19989发酵支原体M.SalivariumATCC23064唾液支原体M.HominisATCC23114人型支原体M.OraleATCC23714口腔支原体M.HyorhinisATCC29052猪鼻支原体其共同[阅读全文:]
摘要:Protocol is for 150 ml of cell culture.1. Spin down cells and wash 1X in 10 ml ice cold PBS.2. Resuspend pellet in 20 ml lysis buffer: 50 mM Tris pH 8.01 % NP-401 mM PMSF1 µg/ml leupeptin3. Tran[阅读全文:]