摘要:DNA测序原理和方法DNA序列测定分手工测序和自动测序,手工测序包括Sanger双脱氧链终止法和Maxam-Gilbert化学降解法。自动化测序实际上已成为当今DNA序列分析的主流。美国PE ABI公司已生产出373型、377型、310型、3700和310[阅读全文:]
摘要:The Solexa sequencing platform comprises all hardware, software, procedures and kitted reagents for DNA sequencing at the whole genome or repetitive regional scale.a) Preparation of full diversity lib[阅读全文:]
摘要:protocols & technical notes DNA Copy Number Analysis Algorithms Infinium Genotyping Data Analysis Using Whole-Genome Amplified DNA Samples in the GoldenGate Genotyping Assay"TOP/BOT" Strand and "A/B" [阅读全文:]
摘要:请下载链接:CSHL_Fu.pdf[阅读全文:]
摘要:请下载链接:Array_Enrichment_AP_f.pdf[阅读全文:]
摘要:Sequences for Solexa Library Preparations: Genomic DNA oligonucleotide sequences Adapters 1 5' P-GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 5' ACACTCTTTCCCTACACGACGCTCTTCCGATCT PCR Primers 1 5' AATGATACG[阅读全文:]
摘要:请下载链接:SOLiD_Brochure.rar[阅读全文:]
摘要:Q:We recently completed a ChIP-seq run on the ABI SOLiD. We received the following data back for one of our samples. These data were from a quarter (1/4) of a slide.------------------61993194 total be[阅读全文:]
摘要:Q:I've been getting mixed messages about the accuracy of the reads coming off the SOLiD platform. A 454 representative swore blind to me last week that only 25% of SOLiD reads are error-free (!), but [阅读全文:]
摘要:Q:my group is involved in a national project to develop a genomic platform for cancer research. while Nimblegen has developed target enrichment chip for the Genome Sequencer 20, nothing similar has be[阅读全文:]