摘要:血浆中的脂类包括胆固醇、胆固醇脂、甘油三酯、磷脂和非酯化脂肪酸等。目前,对有关脂类代谢疾患的诊断和治疗过程,均必须检测血浆(清)中的脂类,通过其含量的变化,对临床疾患提供协助诊断的依据。有关医学科研工作,脂类的定[阅读全文:]
摘要:脂类测定标准化十分重要。心脑血管病的临床和流行病学研究,高脂血症的诊断或治疗过程中血脂变化的监测,以及冠心病危险性的估计都需要准确、精密和实验间可比的测定结果。 一、国外标准化工作的回顾和现状早在1958年,美国[阅读全文:]
摘要:一、目的脂肪广泛存在于许多植物的种子和果实中,测定脂肪的含量,可以作为鉴别其品质优劣的一个指标。脂肪含量的测定有很多方法,如抽提法、酸水解法、比重法、折射法、电测和核磁共振法等。目前国内外普遍采用抽提法,其中索[阅读全文:]
摘要:胆固醇是环戊烷多氢菲的衍生物,它不仅参与血浆蛋白的组成,而且也是细胞的必要结构成分,还可以转化成胆汁酸盐、肾上腺皮质激素和维生素D等。胆固醇在体内以游离胆固醇及胆固醇酯两种形式存在,统称总胆固醇。总胆固醇的测[阅读全文:]
摘要:Contributor: Suprya JayadevDate: November 10, 19931) Prepare a C-18 reverse phase prep-sep cartridge by washing with:a) 10 ml of methanolb) 20 ml of chloroform-methanol (2:1)c) 10 ml of methanold) 10 [阅读全文:]
摘要:OBJECTIVE:Method for incorporating proteins into liposomes for liposome swelling assay or as an alternative antigen presentation method.REAGENTS:Dioleoyl Phosphatidyl CholineBuffer of choice / distill[阅读全文:]
摘要:Contributor: Suprya JayadevDate: Oct. 20, 1994Reagents:2N methanolic NaOH2N HCl--------------------------------------------------------------------------------Protocol:Bligh & Dyer extraction1) Pellet[阅读全文:]
摘要:Contributor: Suprya JayadevDate: Nov. 10, 19931) Incubate cells with 1 µCi/ml of 3H-galactose for 72 hours.---> If treatment is for an extended period of time: treat in serum free media containi[阅读全文:]
摘要:Contributor: Suprya JayadevDate: Mar. 11, 1991I. Lower, chloroform phase:1) Dry on rotovapor system with house vacuum lines. It is not necessary to dry sample completely, but sufficiently to yield a f[阅读全文:]