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基于PCR技术的染色质沉淀分析
词条创建者:admin创建时间:12-06 23:13
标签: 染色质沉淀分析 Chromatin immunoprecitation ChIP

摘要:INTRODUCTION After chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is precipitated at a locus of interest. Real-time PCR amplification is oft[阅读全文:]

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Wholemount Immuno-flurescent
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice Transgenic Identification

摘要:Use 4ml polycarbonate snap-cap tubes for all steps. Rocking of tubes should be performed at all times. All steps are carried out at room temperature unless otherwise stated. Day 0 1. Dissect embryos i[阅读全文:]

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染色质免疫沉淀法(Chromatin immunoprecitation,ChIP)
词条创建者:admin创建时间:12-06 23:13
标签: 染色质沉淀分析 Chromatin immunoprecitation ChIP

摘要:染色质免疫沉淀法(Chromatin immunoprecitation,ChIP)是研究体内DNA与蛋白质相互作用的重要工具。它可以灵敏地检测目标蛋白与特异DNA片段的结合情况,还可以用来研究组蛋白与基因表达的关系。核小体组蛋白可以发生多种[阅读全文:]

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ChIP-CpG Island Microarray Binding Analysis
词条创建者:admin创建时间:12-06 23:13
标签: 染色质沉淀分析 Chromatin immunoprecitation ChIP

摘要:Many methods have been developed to identify genomic targets of DNA-binding factors. Here we outline and discuss our adaptation of the chromatin immunoprecipitation (ChIP) assay to a high-throughput m[阅读全文:]

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ChIP Assay
词条创建者:admin创建时间:12-06 23:12
标签: ChIP Assay

摘要:Formaldehyde cross-linking and chromatin immunoprecipitation assays of tissue culture cells are performed as described by Boyd and Farnham (MCB 29:8389-8399) and Y. Shang et. al. (Cell 103:843-852) wi[阅读全文:]

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染色体组型分析(Karyotype
词条创建者:admin创建时间:12-06 23:04
标签:

摘要:Human chromosome dimensions Chromosome # Relative length Centromere index 1 9.08 48.0 2 8.45 38.1 3 7.06 45.9 4 6.55 27.6 5 6.13 27.4 6 5.84 37.7 7 5.28 37.3 X 5.80 36.9 8 4.96 35.9 9 4.83 33.3 10 4.6[阅读全文:]

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Giemsa-trypsin Banding
词条创建者:admin创建时间:12-06 23:04
标签:

摘要:试剂Earles Balanced Salt Solution (BSS) earlesFetal Bovine SerumGiemsa stain, original azure blend GiemsaHarleco 620G/75 harleco 620g/75Gurrs buffer tablets, pH 6.8 gurrsBiomedical Specialties, Santa [阅读全文:]

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Hybridization for Spectral Karyotyping (SKY)
词条创建者:admin创建时间:12-06 23:04
标签:

摘要:Reagents dH 2OEthanol, absolute (70%, 90%, and 100%)Formamide, deionizedAmbion, Cat. No. 9342HCL, 1NRubber CementSSC, 20X Preparation70% Formamide/2X SSC20X SSC 10 mldH 2O 20 mldeionized formamide 70 [阅读全文:]

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人染色体制备(Human Chromosome Preparation)
词条创建者:admin创建时间:12-06 23:04
标签:

摘要:MaterialsChromosome medium (one bottle per student)--- KaryoMAX Peripheral Blood Karyotyping Medium #41675-018 These bottles usually come in groups of 10 (each bottle conmtaining 5 mL of media).Colcem[阅读全文:]

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DNA提取中的常见问题分析
词条创建者:admin创建时间:12-06 23:03
标签: DNA提取 分析

摘要:提取植物DNA时遇到多糖成分的干扰, DNA质量一直不高,如何消除多糖的影响?参考见解:一般来说,SDS法提取的DNA会还有较多的多糖,使DNA成胶冻状。而CTAB法提取可基本上除去多糖。如果是植物材料本身含糖量比较高,可用以下[阅读全文:]

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遗传学技术