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Micropipette Bevelling
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice Embryo Transfer

摘要:This technique appeared as an article in Biotechniques, Vol.14, No.3, pp412-414. This article is a report by Kristian Gundersen, Theresa Hanley and John Merlie, on the use of micropipette bevelling to[阅读全文:]

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PCR Genotyping from the Embryo Yoke Sac
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice Embryo Transfer

摘要:This method is modified from Dr. Lyn Corcoran (WEHI)Disclaimer: The following set of protocols were contributed by various members of our lab (past and present): Christine Andrews, Fiona Christensen, [阅读全文:]

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PCR Genotyping of Embryos and Blastocysts
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice Embryo Transfer

摘要:These protocols have come from the transgenic mouse list and are intended as a guide, none of them have been tried in this lab Cell(s) transferred in small volume of water to a .5 ml tube and lysed by[阅读全文:]

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Preparation of Microinjection Pipettes
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice Embryo Transfer

摘要:Injection and Holding PipettesThe glass capillary tubing used should be thin walled, borosilicate glass without a fibre. e.g. Clark Electromedical Instruments Australian Agents: SDR Clinical Technolog[阅读全文:]

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Sending Sera to Testing for Mouse Pathogens
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice Embryo Transfer

摘要:Bleed mice: Obtain 300-400µl from mice of various ages (older mice that have been in the room for an extended period) and strains (include nude mice if in the room). 5-8 mice is a good number. A[阅读全文:]

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Vasectomy of Male Mice
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice Embryo Transfer

摘要:Mice are vasectomized to be mated to females used for embryo transfer. The female becomes pseudopregnant, her body geared for a pregnancy because she has been mated. Vasectomy is performed on mice at [阅读全文:]

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Vasectomy of mouse(图)
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice Embryo Transfer

摘要:Mice are vasectomized to be mated to females used for embryo transfer. The female becomes pseudopregnant, her body geared for a pregnancy because she has been mated. Vasectomy is performed on mice at [阅读全文:]

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ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice Gene Transfer

摘要:Need 1.5-2 x 107 cells from a 2 day culture. 1. Cells are harvested as normal, washed x 1 in PBS then taken up at conc. of 1.2 x 10 7 cells/ml in cold PBS. 2. Take x2 Biorad curvettes A. 0.9 ml (107 c[阅读全文:]

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Electroporation of ES cells
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice Gene Transfer ES

摘要:Cells are routinely passaged two days prior to electroporating. Usually one 10 cm plate at approximately 80% confluency will provide enough cells for 1-2 electroporations. Procedure 1. Change medium o[阅读全文:]

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DNA from Tail Biopsies
词条创建者:admin创建时间:12-06 23:13
标签: Transgene Mice Transgenic Identification Tail Biopsies

摘要:1. Remove 0.5 cm of tail into polypropylene microfuge tube (do not mince). (The tubes must have tight-fitting caps, so that there are no leaks in steps 3 and 7 below.) 2. Add 0.5 ml DNA digestion buff[阅读全文:]

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遗传学技术