摘要:一直有同学在尝试流式检测凋亡，一直有同学在问怎么分析大量似是而非或者一塌糊涂的流式“凋亡”数据，一直有同学指着荧光图里的各种各样的“AP”、“subG1”夸夸其谈。关键问题在哪里呢？[阅读全文:]
摘要:C3是由α和β两条肽链通过二硫键连结组成，为β1球蛋白，沉降系数9.5s，分子量180kD，含糖量约占2.2%，是血清中含量最多的补体成分，约占总补体含量的1/3以上，在补体系统激活过程中，无论是经传统途径还是旁路[阅读全文:]
摘要:Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an[阅读全文:]
摘要:Immunoprecipitation buffers and protocols免疫沉淀实验缓冲液及方法Steven Finkbeiner,Departments of Neurology and Physiology,UCSFhttp://gweb1.ucsf.edu/labs/finkbeiner/Protocol/protocol_list/Imm_precip.h[阅读全文:]
摘要:点击浏览该文件 [阅读全文:]
摘要:Chromatin Immunoprecipitation Assay and PCR(galactose inducible genes)adapted by Wendy Reeves (Hahn Lab)from Marnie Gelbart (Tsukiyama lab)Chromatin PreparationTwo days before prep,inoculate 5ml media[阅读全文:]
摘要:Lysis buffer.The choice of lysis buffer depends on what the cells were labeled with and whether you want to obtain an active kinase.The lowest background is obtained with RIPA buffer without EDTA.EDTA[阅读全文:]
摘要:Purpose and backgroundsAbout nuclide...35-SDecay: (b-ray,0.167 MeV)Half life: 87.5 daysThick acrylic shield can block most of the emission.Because of the low energy,it is impossible todetect spillage/[阅读全文:]
摘要:Lysis buffer.The choice of lysis buffer depends on what kind of IP you are doing.RIPA buffer gives the lowest background,but can denature some kinases.It also has the potential to disrupt protein:prot[阅读全文:]
摘要:Hancock Laboratory Methods.Department of Microbiology and Immunology,University of British Columbia,British Columbia,Canadahttp://www.cmdr.ubc.ca/bobh/showmethod.php?methodid=28MATERIALS:Running buffe[阅读全文:]