摘要:Acid Adjusted Swine Serum1. The before or morning of: adjust the pH of the swine serum to between 4.3 and 4.5 with 1N HCl.2. Place serum for at least 1 hour at 4°C (overnight is best).3. Centrifug[阅读全文:]
摘要:1.Yeast (93.75 g) + Super Q Water (562.5 ml) per flask.2.Place in incubator at 37°C for 20 minutes. 3.Heat to 90-100°C for 5 minutes.4.Cool on ice. 5.Pour into centrifuge bottles and centrifug[阅读全文:]
摘要:Base: Bacto Heart Infusion Broth 1.2% Bacto Yeast Extract0.5%Bacto Peptone0.5%NaCl0.025%Na-DNA0.002%pH is adjusted to 8 and autoclaved. May be stored at room temperature for 4 weeks ADD (to final conc[阅读全文:]
摘要:200 ml 1000 ml 4000 ml Mycoplasma broth base (Gibco) 4.5 22.5 90.0 DNA (Sigma, Degraded free acid, type IV) 0.05 0.20 0.80 Glass Distilled Water 142 ml 710 ml 2840 ml Phenol Red (1% aqueous) 0.4 ml 2.[阅读全文:]
摘要:有学者利用离子交换层析和分子筛层析纯化甲型流感病毒。整个过程操作方便，可操作性强，避免了密度梯度离心等操作。Hemagglutinin StabilityStability of HA activity was investigated by titrating inactivated supern[阅读全文:]
摘要:一、目的流感病毒的MDCK细胞分离方法是流感实验的关键技术，用于流感病毒的分离、培养。本SOP是为确保MDCK细胞分离流感病毒的操作准确、规范和可靠而制定。二、范围适用于实验室所有技术人员进行流感病毒的MDCK细胞分离。三、[阅读全文:]
摘要:结核杆菌的培养基的配制网友ljksbs给出结核杆菌培养基配制的方法：结核杆菌培养采用罗－琴培养基，为防止培养基干裂，可加入适量甘油，我们是在1600ml的培养基中加入甘油12ml。罗－琴培养基成分：磷酸二氢钾2.4g；一水硫酸镁0[阅读全文:]
摘要:概述大多数微生物可用超低温保存，超低温保存法是适用范围最广的微生物保存法。需要复杂营养的微生物种，如用其他的贮存方法不能保持其活力（ 如植物的病原性真菌），通常可用超低温的方法保存（ATCC 1983；Halliday，Baker 1[阅读全文:]