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"植物学实验技术" 分类下的词条该分类下有233个词条创建该分类下的词条

DNA isolation & extraction
词条创建者:admin创建时间:12-09 20:06
标签: DNA Extraction Plants DNA提取 植物

摘要:CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolation procedure if both DNA and RNA are needed) Reagents [阅读全文:]

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DNA and RNA EXTRACTIONS
词条创建者:admin创建时间:12-09 20:06
标签: DNA Extraction Plants DNA提取 植物

摘要:A protocol / method / schedule /procedure for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves (See also DNA Isolation protocol) 1) Take one medium sized[阅读全文:]

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Genomic DNA Extraction for Mapping
词条创建者:admin创建时间:12-09 20:06
标签: DNA Extraction Plants DNA提取 植物

摘要:Solutions: Extraction buffer: 200 mM Tris-HCl pH 7.5, 250 mM NaCl, 25 mM EDTA, 0.5% SDS Extraction Buffer1 M Tris-HCl pH 7.510.00 ml5 M NaCl2.50 ml0.5 M EDTA2.50 ml20% SDS1.25 mlH2O33.75 mlTotal50.00 [阅读全文:]

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Rapid DNA Isolation from Phyllanthus Amarus and Other Plant Tissues
词条创建者:admin创建时间:12-09 20:06
标签: DNA Extraction Plants DNA提取 植物

摘要:Procedure Preheat Extraction Buffer at 60°C. Weigh 100 mg of fresh leaf tissue and grind it to powder in Liquid Nitrogen in a chilled mortar and pestle. Add 0.5 ml of TE buffer to the tube. Centri[阅读全文:]

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Arabidopsis RNA extraction protocol
词条创建者:admin创建时间:12-09 20:06
标签: DNA Extraction Plants DNA提取 植物

摘要:Based on Krapp at al (1993) Plant Journal 3:817 with modifications. Comments or Questions? Send to me at: chunming.liu@wur.nl --------------------------------------------------------------------------[阅读全文:]

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Streamlined DNA Extraction Protocol
词条创建者:admin创建时间:12-09 20:06
标签: DNA Extraction Plants DNA提取 植物

摘要:This method is derived from a procedure developed by Toby Bradshaw and the Poplar Molecular Genetics Cooperative. We have tested the procedure with a variety of Populus species, as well as tobacco and[阅读全文:]

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Simplified Arabidopsis Transformation Protocol
词条创建者:admin创建时间:12-09 20:06
标签: Arabidopsis Transformation Plants

摘要:(Brief version for those who are familiar with the method) Steve Clough and Andrew Bent, University of Illinois at Urbana-Champaign. Our present protocol (Clough and Bent, 1998; modified from Bechtold[阅读全文:]

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Competent agro prep for electroporation
词条创建者:admin创建时间:12-09 20:06
标签: Arabidopsis Transformation Plants

摘要:day 1 1. Start 75 mL overnight cultures of agro (strain GV3101 C58C1 Rifr pMP90 Gmr, Koncz & Schell) in YEP in 250 mL baffle flasks. 2. Grow at 28 °C, shaking. 3. Prepare: ~ 3.5 L sterile H2O chil[阅读全文:]

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植物营养器官外部形态与内部结构的观察
词条创建者:admin创建时间:12-09 20:06
标签: 植物 营养器官

摘要:一、实验目的 1.掌握根、根尖的结构,并对根的初生和次生结构进行比较。2.掌握茎的结构,并对单子叶植物茎的初生结构、以及双子叶植物茎的初生和次生结构进行比较。 3.掌握叶的结构,并对单子叶和双子叶植物叶的结构进行比较[阅读全文:]

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Green lab protocol for vacuum infiltration transformation of Arabidopsis
词条创建者:admin创建时间:12-09 20:06
标签: Arabidopsis Transformation Plants

摘要:This protocol is adapted from protocols by Nicole Bechtold (Bechtold et al. 1993), Andrew Bent (Bent et al. 1994) and Takashi Araki. No claims are made that any of the steps are necessary or ideal; th[阅读全文:]

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植物学实验技术