摘要:CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolation procedure if both DNA and RNA are needed) Reagents [阅读全文:]
摘要:Solutions: Extraction buffer: 200 mM Tris-HCl pH 7.5, 250 mM NaCl, 25 mM EDTA, 0.5% SDS Extraction Buffer1 M Tris-HCl pH 7.510.00 ml5 M NaCl2.50 ml0.5 M EDTA2.50 ml20% SDS1.25 mlH2O33.75 mlTotal50.00 [阅读全文:]
摘要:A protocol / method / schedule /procedure for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves (See also DNA Isolation protocol) 1) Take one medium sized[阅读全文:]
摘要:This method is derived from a procedure developed by Toby Bradshaw and the Poplar Molecular Genetics Cooperative. We have tested the procedure with a variety of Populus species, as well as tobacco and[阅读全文:]
摘要:Based on Krapp at al (1993) Plant Journal 3:817 with modifications. Comments or Questions? Send to me at: chunming.liu@wur.nl --------------------------------------------------------------------------[阅读全文:]
摘要:Procedure Preheat Extraction Buffer at 60°C. Weigh 100 mg of fresh leaf tissue and grind it to powder in Liquid Nitrogen in a chilled mortar and pestle. Add 0.5 ml of TE buffer to the tube. Centri[阅读全文:]
摘要:(Brief version for those who are familiar with the method) Steve Clough and Andrew Bent, University of Illinois at Urbana-Champaign. Our present protocol (Clough and Bent, 1998; modified from Bechtold[阅读全文:]
摘要:day 1 1. Start 75 mL overnight cultures of agro (strain GV3101 C58C1 Rifr pMP90 Gmr, Koncz & Schell) in YEP in 250 mL baffle flasks. 2. Grow at 28 °C, shaking. 3. Prepare: ~ 3.5 L sterile H2O chil[阅读全文:]
摘要:This protocol is adapted from protocols by Nicole Bechtold (Bechtold et al. 1993), Andrew Bent (Bent et al. 1994) and Takashi Araki. No claims are made that any of the steps are necessary or ideal; th[阅读全文:]