摘要:Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases [阅读全文:]
摘要:This method is derived from a procedure developed by Toby Bradshaw and the Poplar Molecular Genetics Cooperative. We have tested the procedure with a variety of Populus species, as well as tobacco and[阅读全文:]
摘要:一、DNA 提取 SDS 高盐法(方案1) 具体步骤: 称取1 g 土壤，放入研钵中，倒入适量的液氮，立即研磨；再倒入适量的液氮，研磨，重复3 次，使土壤颗粒研成粉末； 将13.5 ml 提取缓冲液(0.1 mol/L磷酸盐[pH = 8.0 ] ，0.1 mol/LEDT[阅读全文:]
摘要:Materials: 1.TENS solution: 10 mM Tris (pH to 7.5) 1 mM ethylenediaminetetraacetic acid (pH to 8.0 to dissolve) 0.1 N sodium hydroxide 0.5 % sodium dodecyl sulfate2.3 M Sodium acetate, pH 5.2 3.Pre-ch[阅读全文:]
摘要:1.Obtain 65-100 µl of blood by retro-orbital bleed with a heparinized microcapillary tube. Expel blood immediately into a 1.5 ml microfuge tube containing 20 µl of 10 mM EDTA. Mix immediat[阅读全文:]
摘要:1. Remove 0.5 cm of tail into polypropylene microfuge tube (do not mince).(The tubes must have tight-fitting caps, so that there are no leaks in steps 3 and 7 below.)2. Add 0.5 ml DNA digestion buffer[阅读全文:]
摘要:1. Transfer cells to a 15 ml tube.2. Add 5 ml TBS- to flasks, shake & pour into tubes.3. Spin down the cells @ 1K RPM for 5'.4. Resuspend in 1 ml TBS-.5. Transfer to 1.5 ml tubes.6. Spin down @ 14K RP[阅读全文:]
摘要:CTAB TECHNIQUE / Method / Schedule / Protocol (JPB) FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES(see also DNA RNA double isolation procedure if both DNA and RNA are needed)Reage[阅读全文:]
摘要:Preparation of BAC (Bacterial Artificial Chromosome) DNA with CONCERT ™ High Purity Plasmid Purification System1 Courtesy Lisha Xu and Alice C. Young, Molecular and Cell Biology Research and Dev[阅读全文:]
摘要:The following protocol is based on our modifications of R. Kraft, J. Tardiff, K. S. Krauter, and L. A. Leinwand. Biotechn . 6(6):544-545, 1988.Inoculate 2-5 ml of L broth containing the appropriate an[阅读全文:]