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Oligonucleotide?Purification
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摘要:Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases [阅读全文:]

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Streamlined?DNA?Extraction?Protocol
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摘要:This method is derived from a procedure developed by Toby Bradshaw and the Poplar Molecular Genetics Cooperative. We have tested the procedure with a variety of Populus species, as well as tobacco and[阅读全文:]

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提取土壤总DNA的几种方法
词条创建者:admin创建时间:12-09 23:23
标签: 土壤总DNA

摘要:一、DNA 提取 SDS 高盐法(方案1) 具体步骤: 称取1 g 土壤,放入研钵中,倒入适量的液氮,立即研磨;再倒入适量的液氮,研磨,重复3 次,使土壤颗粒研成粉末; 将13.5 ml 提取缓冲液(0.1 mol/L磷酸盐[pH = 8.0 ] ,0.1 mol/LEDT[阅读全文:]

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Plasmid?DNA?Isolation?from?Bacteria
词条创建者:admin创建时间:12-09 23:23
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摘要:Materials: 1.TENS solution: 10 mM Tris (pH to 7.5) 1 mM ethylenediaminetetraacetic acid (pH to 8.0 to dissolve) 0.1 N sodium hydroxide 0.5 % sodium dodecyl sulfate2.3 M Sodium acetate, pH 5.2 3.Pre-ch[阅读全文:]

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DNA?From?Whole?Blood?for?PCR
词条创建者:admin创建时间:12-09 23:23
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摘要:1.Obtain 65-100 µl of blood by retro-orbital bleed with a heparinized microcapillary tube. Expel blood immediately into a 1.5 ml microfuge tube containing 20 µl of 10 mM EDTA. Mix immediat[阅读全文:]

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DNA?from?Tail?Biopsies
词条创建者:admin创建时间:12-09 23:23
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摘要:1. Remove 0.5 cm of tail into polypropylene microfuge tube (do not mince).(The tubes must have tight-fitting caps, so that there are no leaks in steps 3 and 7 below.)2. Add 0.5 ml DNA digestion buffer[阅读全文:]

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制备BAC用DNA
词条创建者:admin创建时间:12-09 23:23
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摘要:Preparation of BAC (Bacterial Artificial Chromosome) DNA with CONCERT ™ High Purity Plasmid Purification System1 Courtesy Lisha Xu and Alice C. Young, Molecular and Cell Biology Research and Dev[阅读全文:]

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CAT?ASSAY?(liquid?phase)
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摘要:1. Transfer cells to a 15 ml tube.2. Add 5 ml TBS- to flasks, shake & pour into tubes.3. Spin down the cells @ 1K RPM for 5'.4. Resuspend in 1 ml TBS-.5. Transfer to 1.5 ml tubes.6. Spin down @ 14K RP[阅读全文:]

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CTAB TECHNIQUE / Method / Schedule / Protocol (JPB) FOR DNA
词条创建者:admin创建时间:12-09 23:23
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摘要:CTAB TECHNIQUE / Method / Schedule / Protocol (JPB) FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES(see also DNA RNA double isolation procedure if both DNA and RNA are needed)Reage[阅读全文:]

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PREPARATION OF PLASMID DNA FOR SEQUENCING
词条创建者:admin创建时间:12-09 23:23
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摘要:The following protocol is based on our modifications of R. Kraft, J. Tardiff, K. S. Krauter, and L. A. Leinwand. Biotechn . 6(6):544-545, 1988.Inoculate 2-5 ml of L broth containing the appropriate an[阅读全文:]

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DNA技术