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Pyrosequencing 在肿瘤基因甲基化研究的应用
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摘要:Pyrosequencing 在肿瘤基因甲基化研究的应用 甲基化研究对于基因的调控和肿瘤的发生有非常密切的关系。在一般正常的细胞中,基因调控区CpG岛处于非甲基化的状态。而当细胞发生癌变后,这些CG区域往往呈现甲基化状态。英国[阅读全文:]

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sothing about Genome walking
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摘要:Can anyone recommend a good kit for genome walking? I would like to find out the promoter sequence of a known gene. Is genome walking the way to do it?thanks a million for any suggestions!-kiwi-Kiwi-I[阅读全文:]

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Genome Walking Kit使用注意
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摘要:用于Genome Walking Kit的模板总DNA 一般要满足以下几点:1、DNA 的完整性,电泳为单一条带。2、避免杂质,OD260/280=1.8~2.0。3、模板DNA 不要被污染。4、准确定量,不要少于3μg。[阅读全文:]

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Genomes
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摘要:Publisher: Bios Scientific Publishers LtdNumber Of Pages: 608Publication Date: 2002-06-15Sales Rank: 4476658ISBN / ASIN: 1859962289EAN: 9781859962282Binding: HardcoverManufacturer: Bios Scientific Pub[阅读全文:]

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Preparation of BAC DNA with by Alkaline Prep Method
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摘要:CLONE ISOLATION PREP - PROTOCOLBefore you begin isolating DNA from the cultures:--GENTLENESS is key through step 10!--Try to do prep steps 1-8 at the side of the centrifuge--Make Solution III and plac[阅读全文:]

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Genomic?DNA?extraction
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摘要:点击浏览该文件[阅读全文:]

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MITOCHONDRIAL?DNA?ISOLATION
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摘要:ProcedureGrind in mortar and pestle or Waring blender with 5-7 volumes buffer A per g tissue. Use MCE at 350 l/L, and if necessary, with 5 ml 1 M DIECA/L. Squeeze through cheesecloth, two layers of Mi[阅读全文:]

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ES?CELL?DNA?EXTRACTION:?TUBE?ME
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摘要:Protocol for extracting DNA from ES Cells, starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate thi[阅读全文:]

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Preparation?of?G+A?Marker
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摘要:Author: Long-Cheng Li Source: Protocol Online Abstract: Simplified method for preparing G+A ladder run along with footprinting reaction. It's much simple than the original Maxima-Gilbert sequencing re[阅读全文:]

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CAT?ASSAY[TLC?METHOD]
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摘要:CAT ASSAY[TLC METHOD] 1. Transfer cells to a 15 ml tube. 2. Add 5 ml TBS- to flasks, shake & pour into tubes. 3. Spin down the cells @ 1k rpm for 5'. 4. Resuspend in 1 ml TBS- . 5. Transfer to 1.5 ml [阅读全文:]

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DNA技术