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"DNA技术" 分类下的词条该分类下有1132个词条创建该分类下的词条

CAT?ASSAY[TLC?METHOD]
词条创建者:admin创建时间:12-09 23:22
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摘要:CAT ASSAY[TLC METHOD] 1. Transfer cells to a 15 ml tube. 2. Add 5 ml TBS- to flasks, shake & pour into tubes. 3. Spin down the cells @ 1k rpm for 5'. 4. Resuspend in 1 ml TBS- . 5. Transfer to 1.5 ml [阅读全文:]

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Spin?Column?chromatography
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摘要:Used to removed unincorporated nucleotides from labelling reactions.Prepare Sephadex G-50 (medium) by adding appropriate amount of dry beads to 100 ml TE buffer such that the beads will swell to 50 ml[阅读全文:]

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寡核苷酸纯化
词条创建者:admin创建时间:12-09 23:22
标签: 寡核苷酸

摘要:Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases [阅读全文:]

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Ethanol?Precipitation?of?DNA
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摘要:This procedure allows the concentration of DNA samples from dilute solution and the removal of unwanted salts from DNA samples.Materials 3M Sodium Acetate buffer, pH 5.2 (store at 4 ℃). Cold 100% Etha[阅读全文:]

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DNA?Preparation?from?Cell?Lines
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摘要: 点击浏览该文件 [阅读全文:]

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Dialysis?(using?dialysis?tubing)
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摘要:Molecularpourous membrane tubing is used for desalting, protein and DNA isolation / purification. It comes in several diameters and pore sizes (for molecular weight conversions for nucleic acids see g[阅读全文:]

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琼脂糖凝胶DNA纯化回收试剂盒--Promega
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摘要:Promega WizardSV Gel and PCR Clean-up System 品牌:Promega回收范围:100bp-10kb价格:(50次包装的试剂盒报价为562元,11元/次)Promega的产品在进口品牌中一向以价格可亲而著称。这个胶回收试剂盒也不例外。并且这个试[阅读全文:]

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MINI?PREP?BY?BOILING
词条创建者:admin创建时间:12-09 23:22
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摘要:Pellet 1.5 ml of an overnight culture at 12,000 rpm in Eppendorf centrifuge at RT for 3 min. Resuspend bacterial pellet in 350 µl of STET buffer. Add 25 µl of freshly prepared solution of [阅读全文:]

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Gene-Kleen?protocol
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摘要: Purification of DNA from agarose gels is an essential method involved in the sub-cloning of DNA fragments. The following method describes a variation of the method of Vogelstein and Gillespie, 1979 ([阅读全文:]

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DNA?labeling?by?nick?translation
词条创建者:admin创建时间:12-09 23:22
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摘要:reagents: DNA for labeling (concentration c > 150 ng/µl) modified nucleotides: Biotin-16-dUTP, Digoxigenin-11-dUTP, conc. 1nmol/µl (Boehringer Mannheim) dNTPs (regular nucleotides): dATP, [阅读全文:]

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DNA技术