摘要: Prepare a ligation mix:Ligation Mix (2x)10x ligase buffer 1.0 ml digested vector(0.1 mg/ml) 1.0 ml H2 O 6.0 ml total 8.0 ml divide ligation mix into two Eppendorf tubesLigation RxnInsert Control liga[阅读全文:]
摘要:所谓亚克隆就是对已经获得的目的DNA片段进行重新克隆，其目的在于对目的DNA进行进一步分析，或者进行重组改造等。亚克隆的基本过程包括：(1)目的DNA片段和载体的制备；(2)目的DNA片段和载体的连接；(3)连接产物的转化；(4)[阅读全文:]
摘要:Phosphatase treatment of DNA fragments .pdf[阅读全文:]
摘要:平端连接通常情况下，PCR产物可直接与平端载体DNA进行连接，但其连接效率效低。因为TaqDNA聚合酶具有非模板依赖性末端转移酶活性，能在两6条DNA链的3'末端加上一个多余的碱基，使合成的PCR产物成为3'突出一个碱基的DNA分[阅读全文:]
摘要:一、克隆的早期研究 克隆一词是英文单词clone的音译，作为名词，c1one通常被意译为无性繁殖系。同一克隆内所有成员的遗传构成是完全相同的，例外仅见于有突变发生时。自然界早已存在天然植物、动物和微生物的克隆，例如：同卵[阅读全文:]
摘要:什么是克隆？克隆是英语单词clone的音译，clone源于希腊文klone，原意是指幼苗或嫩枝，以无性繁殖或营养繁殖的方式培育植物，如杆插和嫁接。如今，克隆是指生物体通过体细胞进行的无性繁殖，以及由无性繁殖形成的基因型完全[阅读全文:]
摘要:Use of oligonucleotides in various research applications requires certain basic storage and handling techniques in order to ensure trouble-free experiments. Proper storage of your oligonucleotide will[阅读全文:]
摘要:Source: Protocol OnlineAbstract: Modifying DNA using sodium bisulfite to convert unmethylated cytosines to uracils and subsequently detect methylated cytosines using methylation specific PCR (MSP) tec[阅读全文:]
摘要:DNA methylation is an epigenetic event that affects cell function by altering gene expression and refers to the covalent addition of a methyl group, catalyzed by DNA methyltransferase (DNMT), to the 5[阅读全文:]
摘要:Materials:3 M sodium acetate pH 5.2 or 5 M ammonium acetateDNA100% ethanolMeasure the volume of the DNA sample. Adjust the salt concentration by adding 1/10 volume of sodium acetate, pH 5.2, (final co[阅读全文:]