摘要:1 基因芯片技术分离目的基因生物芯片是高密度固定在固相支持介质上的生物信息分子的微列阵。列阵中每个分子的序列及位置都是已知的，并按预先设定好的顺序点阵。基因芯片是生物芯片的一种，其上固定的是核算类物质，主要[阅读全文:]
摘要:DNA WALKING REFERRENCEKi-Bum Park and Suk-Heung Oh(2007)Cloning, sequencing and expression of a novel glutamate decarboxylase gene from a newly isolated lactic acid bacterium, Lactobacillus brevis OPK[阅读全文:]
摘要:1. BACs are usually supplied as stabs in agar (2.5 ml screw-top tubes filled with LB agar, stabbed with a toothpick which has been put into a bacterial colony). These are then frozen; at -70℃ they wil[阅读全文:]
摘要:The following guidelines should be taken into account when designing modified oligonucleotides. 1.Sequence Length - SYNTHEGEN can synthesize oligonucleotides from 5 to 110 bases in length. Most sequen[阅读全文:]
摘要:FINGERPRINTING PROTOCOL (AGAROSE GEL) * Restriction digests consist of:15.75 ml ddH2 O 1 µl 10 X buffer B (Boehringer Mannheim) 0.25 µl HindIII (40 U/µl) 3 µl DNA Set up digest[阅读全文:]
摘要: An end labeled DNA probe is incubated with a purified DNA-binding factor or with a protein extract. The unprotected DNA is then digested with DNase I such that on average, every DNA molecule is cut o[阅读全文:]
摘要:Protocol:Combine an appropriate concentration of probe that has been labeled on one strand (100-150 fmol probe has worked so far) with the appropriate concentration of protein in a total volume of 50&[阅读全文:]
摘要:Protocol For the original protocol describing CGH see the article by Kallioniemi et al . For an example of how this technique has been utilized by the NCI Prostate Cancer Working Group see the section[阅读全文:]
摘要:This technique can be used to isolate overlapping DNA fragments starting with a previously cloned DNA fragment that maps near a gene of interest (dark red). The walk is continued until a clone contain[阅读全文:]
摘要:一、导论 质粒提取的原理:转自复旦大学一位老师的帖子，后面是方法介绍 碱裂解法从大肠杆菌制备质粒，是从事分子生物学研究的实验室每天都要用的常规技术。可是我收研究生十几年了，几乎毫无例外的是我那些给人感觉什么都[阅读全文:]