摘要:石蜡包埋组织的DNA提取及其应用 近10年来，现代分子生物学技术越来越广泛地被用于人类疾病研究的诸领域，为了解病理状态下基因组DNA的变化积累了新资料。目前认为，人类基因组并非人们想像的那样稳定，诸如基因重排、扩增、[阅读全文:]
摘要:1、Grow an overnight culture from a single colony in 2 mls LB + antibiotic.2、Transfer 1.5 mls of culture to eppendorf tube.Spin for 1 minute on high and remove supernatant.3、Add 700 µl STET. Add [阅读全文:]
摘要: 1、Put a 0.5-1.5 CM MOUSE TAIL SNIP in a 1.5 ml eppindorf tube and store frozen until digestion.Make certain that the eppindorf tubes seal well,otherwise your samples may leak out during the shaking s[阅读全文:]
摘要:Sample DNAs should not be highly degraded nor contain PCR inhibitors, such as high concentrations of heme or chelating agents. For each individual assayed, 250 ng of genomic DNA are digested separatel[阅读全文:]
摘要:1.Prepare or obtain Buffered phenol,pH 8.0.Add 2 crystals of 8-Hydroxyquinoline to prevent oxidation.This also identifies the organic phase as yellow-colored.2.Combine DNA sample with an equal volume [阅读全文:]
摘要:ReagentsChloroformEDTA,0.5 MEthanol, absoluteIsoamyl alcoholSigma,Cat.I-3643PhenolPhosphate Buffered Saline(PBS),1XProteinase KEM Science,Gibbstown,WV Cat.24568-2(100 mg)RNase ABoehringer Mannheim,Cat[阅读全文:]
摘要:一、碱变性法提取质粒DNA 质粒(Plasmid)是细菌染色体外能自身独立复制的双股环状DNA。带有遗传信息，可赋予细菌某些新的表型。将质粒指纹图谱分析方法、质粒DNA探针技术及检测质粒的PCR技术用于临床感染性疾病的诊断和流[阅读全文:]
摘要:Grind in mortar and pestle or Waring blender with 5-7 volumes buffer A per g tissue.Use MCE at 350 l/L,and if necessary,with 5 ml 1 M DIECA/L.Squeeze through cheesecloth, two layers of Miracloth,10 mi[阅读全文:]
摘要:There are many methods for DNA preparation without using kits.Below are a few minipreps.These protocols may be scale up to midi or maxi prep if necessary.1.Ammonium Acetate Method 1)Spin down cell and[阅读全文:]