摘要:IntroductionMethods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the internet. A commonly occurring theme on [阅读全文:]
摘要:Phenol (removes protein)1、add equal volume of Phenol (= tris-saturated Phenol-Chloroform-Isoamyethanol)2、vortex3、spin 2 minutes at 12000 rpm 4℃4、transfer supernatant to a fresh tube (avoid aspiration [阅读全文:]
摘要:1. Spin down 1.5 ml of overnight culture in 2ml or 1.5ml tube for 1 minute on high. 2. Asprirate supernatant and resuspend cell pellet in 100 µl Solution I (25 mM Tris-HCl, pH 8.0, 10 mM EDTA). [阅读全文:]
摘要:Equipment and reagents1.Phenol2. TE buffer,pH 8.0 (10 mM Tris-HCl,pH 8.0;1 mM EDTA,pH 8.0)3. 24:1 (v/v) chloroform-isoamyl alcohol4. 3 M potassium acetate, pH 5.5,prepared by adding glacial acetic aci[阅读全文:]
摘要:1. Add an equal volume (equal to sample volume) of P/C to sample.2. Mix (shake, don't vortex).3. Take aqueous (upper) layer. (If dirty sample, repeat Ph/Chl step until interface is fairly clean).4. Ad[阅读全文:]
摘要:Add NH4Ac (10 M stock or solid) to the sample for a final concentration of 2.5 M, mix (spin at 4℃, transfer the supernatant to a new tube; optional spin for extra purification of the DNA), add 2.5 vol[阅读全文:]
摘要:1. Separate DNA fragments in an agarose gel cast with 0.5 mg/ml Ethidium bromide. Locate bands with a hand-held long-wave UV lamp.2. Slice the gel with a razor blade above and below the bands of inter[阅读全文:]
摘要:1) Take one medium sized leaf or half a large leaf (5 to 20 cm^2), weigh and freeze in liquid nitrogen.2) Grind the tissue in a bleached and baked pestle and mortar with liquid nitrogen.3) Transfer th[阅读全文:]
摘要:琼脂糖凝胶作为核酸检测主要也是理想材料，使用十分广泛，其理化性质一些参数如下表： （琼脂糖是琼脂内非离子型的组分，它在 0 ~ 4 ℃， pH 4~9 范围内是稳定的。） 名称、型号 凝胶内琼脂糖百分含量（ W/W ）排阻的下限（ Mr ）[阅读全文:]
摘要:Wear gloves throughout and work in radiation area.Monitor area before and after use.Mix the following in an eppendorf tube:1、0.5 microgram oligonucleotide dissolved in H2O.2、3 microliters 10x kinase b[阅读全文:]