摘要:Oligo designThe 5' end (the homology arm) - choose 42 or more (we usually choose about 50) nts for the homology arms from the target DNA sequence simply according to where you want to insert the PCR f[阅读全文:]
摘要:Protocols for HapMap assay-design Affymetrix platform (used by Broad)Defined protocols:LSID:urn:LSID:affymetrix.hapmap.org:Protocol:affy_assay_design_1:1Title: Genotyping using Affymetrix arraysDescri[阅读全文:]
摘要:TA Cloning exploits the terminal transferase activity of some DNA polymerases such as Taq polymerase. This enzyme adds a single, 3'-A overhang to each end of the PCR product. This makes it possible to[阅读全文:]
摘要: The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters a[阅读全文:]
摘要:FootprintingFootprinting is a method for determining the exact DNA sequence to which a particular DNA-binding protein binds. Examples: hormone-receptor complexes that bind to their hormone response el[阅读全文:]
摘要: 点击浏览该文件[阅读全文:]
摘要:一、导论已经提出过许多方法用于从细菌中提纯质粒DNA ， 这些方法都含有以下3个步骤：细菌培养物的生长。细菌的收获和裂解质粒DNA 的纯化。（一）细菌培养物的生长从琼脂平板上挑取一个单菌落，接种到培养物中( 有含有行当抗[阅读全文:]
摘要:DNaseI FootprintintSolutions10X Binding Buffer200 mM Tris 8.0 200 m l 1M Tris pH 8.0500 mM NaCl 100 m l 5M NaCl10 mM EDTA 20 m l 0.5 M EDTA pH 8.0680 m l Qstore at room temperatureDNaseI Dilution Buff[阅读全文:]
摘要:1. Add 400 μl of TE buffer then 400 μl of 1-butanol to the oligonucleotide glass vial.2. Vortex well, then spin down in tabletop centrifuge for 10 minutes at ~2,000. rpm¹s.3. Remove top, bu[阅读全文:]
摘要:This protocol was designed to generate directionally end-labeled probes for DNaseI footprinting but it can be used for any application that requires end-labeled DNA probes.Solutions10 mM dNTP StocksTh[阅读全文:]