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RNA干扰技术获得新突破
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摘要:来自一家名为“ALNYLAM”的生物技术公司的研究人员在11日出版的《自然》杂志上报告说,他们通过转基因技术 在RNA(核糖核酸)干扰技术的研究上取得了突破,为治疗糖尿病 、癌症等疾病带来了希望。RNA干扰是一种由[阅读全文:]

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目前已发表文章中RNAi设计的片段综述
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摘要: Gene (Ref) [阅读全文:]

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ESTs?from?Phage?Plaques
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摘要:The Queensland Institute for Medical Research (QIMR) lab's method for automated sequencing for expressed sequence tags from Schistosoma japonicum and/or S. mansoni cDNA libraries constructed in the di[阅读全文:]

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RNA Extraction--RNA提取
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摘要: All solutions used in RNA preparation should be treated with DEPC as follows: add DEPC as follows: add DEPC to 0.1% and vigorously stir for one hour to O/N followed by autoclaving for 30 minutes. Bak[阅读全文:]

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RNA提取--RNA ISOLATION
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摘要: DEP treatment solutions. Add 0.3 ml lysing buffer (0.1 M Hepes, 0.2 M EDTA, 10% SDS, 0.02 M EGTA (pH 7.5) [DEP-treated) to 3 ml bacterial solution. Extract with 1 vol 1000:140:0.4 (w:w:w) phenol:m-cr[阅读全文:]

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Isolation of total RNA using CsCl/EtBr gradients--密度梯度法提取总RNA
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摘要:RNA for S1 or PE analysis must be free of chromosomal DNA.This can be accomplished by extraction of the RNA with hot phenol, but phenol can, especially if its pH is acidic, cause the specific loss of [阅读全文:]

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细菌RNA的提取--ISOLATION OF RNA FROM BACTEROIDS
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摘要:3 g nodules (fresh or frozen in liquid N2) were ground to a powder in mortar and pestle with liquid N2. To the powder was added ice cold 0.5 M mannitol (or sucrose), 0.05 M Tris (pH 7.5), 0.02 M Na su[阅读全文:]

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植物RNA提取--Methods for Plant RNA Isolation
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摘要: AMES/Chloroform extraction in our lab to extract total from potato leaves for viroid analysis. The protocol is as follows:AMES Buffer (for 200mL): 11.7g NaCl160 mL dH2OAdjust pH to 6.00.40g MgCl28.21[阅读全文:]

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Secondary structure of prion mRNA
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摘要:Secondary structure of prion mRNALuck R; Steger G; Riesner D Institut fur Physikalische Biologie, Heinrich-Heine-Universitat, Dusseldorf, FRG.J Mol Biol 258: 813-26 (1996) An algorithm for prediction [阅读全文:]

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SINGLE STEP PROKARYOTIC RNA ISOLATION
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摘要:In the past, poly(A)+ RNA has not been detected in prokaryotes.In the early 80s, a new method of isolating RNA from bacteria was developed, involving lysis by protease K in the presence of SDS and pot[阅读全文:]

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RNA技术