摘要:Prior to Extractions ? Bake all necessary glassware, metal spatulas, mortars, and pestles overnight in a 200℃ oven afterwrapping them in aluminum foil. For each sample, you should minimally prepare on[阅读全文:]
摘要:1. Snap freeze ~107 cells or ~100 mg of tissue in liquid nitrogen or dry ice/ethanol.2. Transfer the frozen sample to a mortar and pestle and grind to a fine powder. Or use a hand-held tissue grinder [阅读全文:]
摘要: Reagents and EquipmentsTRIzol Reagent (Life Technologies cat# 15596-026) or TRI reagent (Sigma cat # T-9424)DEPC (RNase free) water or 0.5% SDS solution in DEPC treated waterChloroform (Fisher )Isopr[阅读全文:]
摘要:The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by the discovery and characterization of DNA-dependant [阅读全文:]
摘要: Restriction Map and Cloning Site of the RNAi-Ready pSIREN-DNR Vector. Unique restriction sites are in bold. RNAi-Ready pSIREN-DNR is provided as a linearized vector digested with BamH I and EcoR I. N[阅读全文:]
摘要:RNAi相关文章（for free）Prospects of RNA interference therapy for cancerS I Pai, Y-Y Lin, B Macaes, A Meneshian, C-F Hung and T-C WuGene Ther 13: 464-477; advance online publication, December 8, 2005; d[阅读全文:]
摘要:The Easiest Route to Guaranteed SilencingIncreasing numbers of researchers are using small interfering RNAs (siRNAs) to reduce the expression of specific mammalian genes. Applications of siRNA induced[阅读全文:]
摘要:1.相似性序列发生Cross－react引起脱靶效应。2.监测手段：对脱靶效应的监测一般通过基因芯片来监测。Gene expression profiling对脱靶效应。3.脱靶效应的特性：转录物表达模式有序列特异性和靶特异性两种。4.大部分脱靶效应[阅读全文:]
摘要:Post-transcriptional gene silencing (PTGS), which was initially considered a bizarre phenomenon limited to petunias and a few other plant species, is now one of the hottest topics in molecular biology[阅读全文:]
摘要:Background Information of RNA iRNA interference (RNA i) is a biological process in which the introduction of double-stranded RNA (dsRNA ) into a cell results in targeted post-tranional gene silencing.[阅读全文:]