生命经纬知识库>> 分类 >> 实验技术 >> RNA技术

"RNA技术" 分类下的词条该分类下有246个词条创建该分类下的词条

RNA purification --- Trizol
词条创建者:admin创建时间:12-09 22:51
标签:

摘要:1. Snap freeze ~107 cells or ~100 mg of tissue in liquid nitrogen or dry ice/ethanol.2. Transfer the frozen sample to a mortar and pestle and grind to a fine powder. Or use a hand-held tissue grinder [阅读全文:]

编辑:0次| 浏览:1119次
Microscale Hot Borate RNA Extraction from Cotton Tissue--从棉花组织中提取微量RNA
词条创建者:admin创建时间:12-09 22:51
标签:

摘要:Prior to Extractions ? Bake all necessary glassware, metal spatulas, mortars, and pestles overnight in a 200℃ oven afterwrapping them in aluminum foil. For each sample, you should minimally prepare on[阅读全文:]

编辑:0次| 浏览:2455次
RNA purification using TRizol--TRIZOL法提取RNA
词条创建者:admin创建时间:12-09 22:51
标签:

摘要: Reagents and EquipmentsTRIzol Reagent (Life Technologies cat# 15596-026) or TRI reagent (Sigma cat # T-9424)DEPC (RNase free) water or 0.5% SDS solution in DEPC treated waterChloroform (Fisher )Isopr[阅读全文:]

编辑:0次| 浏览:1558次
RNase?Protection?Assay
词条创建者:admin创建时间:12-09 22:51
标签:

摘要:The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by the discovery and characterization of DNA-dependant [阅读全文:]

编辑:0次| 浏览:982次
RNAi载体pSIREN-DNR?Vector
词条创建者:admin创建时间:12-09 22:51
标签:

摘要: Restriction Map and Cloning Site of the RNAi-Ready pSIREN-DNR Vector. Unique restriction sites are in bold. RNAi-Ready pSIREN-DNR is provided as a linearized vector digested with BamH I and EcoR I. N[阅读全文:]

编辑:0次| 浏览:1072次
RNAi相关文献资料
词条创建者:admin创建时间:12-09 22:51
标签:

摘要:RNAi相关文章(for free)Prospects of RNA interference therapy for cancerS I Pai, Y-Y Lin, B Macaes, A Meneshian, C-F Hung and T-C WuGene Ther 13: 464-477; advance online publication, December 8, 2005; d[阅读全文:]

编辑:0次| 浏览:858次
The?Easiest?Route?to?Guaranteed
词条创建者:admin创建时间:12-09 22:51
标签:

摘要:The Easiest Route to Guaranteed SilencingIncreasing numbers of researchers are using small interfering RNAs (siRNAs) to reduce the expression of specific mammalian genes. Applications of siRNA induced[阅读全文:]

编辑:0次| 浏览:820次
RNAi off-target 效应
词条创建者:admin创建时间:12-09 22:51
标签:

摘要:1.相似性序列发生Cross-react引起脱靶效应。2.监测手段:对脱靶效应的监测一般通过基因芯片来监测。Gene expression profiling对脱靶效应。3.脱靶效应的特性:转录物表达模式有序列特异性和靶特异性两种。4.大部分脱靶效应[阅读全文:]

编辑:0次| 浏览:1049次
Background Information of RNAi
词条创建者:admin创建时间:12-09 22:51
标签:

摘要:Background Information of RNA iRNA interference (RNA i) is a biological process in which the introduction of double-stranded RNA (dsRNA ) into a cell results in targeted post-tranional gene silencing.[阅读全文:]

编辑:0次| 浏览:844次
RNAi和基因沉默的历史回顾
词条创建者:admin创建时间:12-09 22:51
标签:

摘要:Post-transcriptional gene silencing (PTGS), which was initially considered a bizarre phenomenon limited to petunias and a few other plant species, is now one of the hottest topics in molecular biology[阅读全文:]

编辑:0次| 浏览:1064次
 共246条 ‹‹12345678910››... 25

RNA技术