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RNase Protection Assay
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摘要: RNase Protection Assay (RPA) BD Biosciences公司, PDF文件http://www.bdbiosciences.ca/canada/downloads/protocols/RPA.pdf[阅读全文:]

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RNase Protection Assay--RNA酶保护试验
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摘要: RNA酶保护试验(RNase Protection Assay,RPA)是通过液相杂交的方式,用反义RNA探针与样品杂交,以检测RNA表达的技术。与Northern杂交和RT-PCR比较,RPA有以下几个优点:1. 检测灵敏度比Northern杂交高。由于Northern杂交步[阅读全文:]

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Primer Extension analysis of total yeast RNA
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摘要:Primer Extension analysis of total yeast RNA 1. Mix the following in a 1.5 ml eppendorf tube:a. 20-80 micrograms yeast RNA dissolved in H2O (the amount will depend on the level of expression for the g[阅读全文:]

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Ribonuclease A (RNase A), DNase-free
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摘要:FeatureThe RNase A is free of DNase activity. It is not necessary to heat it before use.DescriptionThe Ribonuclease A (RNase A) is an endoribonuclease that specifically degrades single-stranded RNA at[阅读全文:]

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RNAi原理FLASH演示
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摘要:How does RNAi work? Genetic and biochemical data indicate a possible two-step mechanism for RNA interference (RNAi): an initiation step and an effector step. a | In the first step, input double-strand[阅读全文:]

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RNAi实验中双链短RNA(dsRNA)制备过程
词条创建者:admin创建时间:12-09 22:50
标签: 双链短RNA

摘要:RNAi 实验中双链短RNA(dsRNA)制备过程,本实验方法来自于加州大学Jim教授实验,很权威!Procedure for the Generation of dsRNA for use in RNAi1. Design polymerase chain reaction (PCR ) primers that will amplify[阅读全文:]

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RNAi术语表
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摘要:RNA i Glossary Dicer - Dicer is a member of the RNase III family of nucleases that specifically cleave double-stranded RNA s. Dicer processes long dsRNA into siRNA of 21-23 nt. Interferon - A small an[阅读全文:]

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shRNA 资源库及其构建方法
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摘要:1.NCBIshRNA资源库 http://cgap.nci.nih.gov/RNAi About RNAi on CGAPThe NCBI is part of the consortium supporting the preparation of human and mouse libraries containing RNAi constructs that target cance[阅读全文:]

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RNAi在植物中的应用
词条创建者:admin创建时间:12-09 22:50
标签: RNAi

摘要:RNA沉默是广泛存在于生物中的一种古老现象,是生物抵抗异常DNA的一种保护机制,同时在生物生长发育过程中扮演着基因表达调控的角色。文章对RNA沉默研究中的几个热点问题进行了介绍,按RNA沉默持续时间的不同对其在植物中[阅读全文:]

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The pSico and pSicoR Design and Cloning
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摘要:pSico Oligo Design Click on the Mac OSX program pSicoOligomaker 1.5 to select and design oligos for pSico and plentilox3.7.To operate it, simply paste your sequence in the "sequence" window, give it a[阅读全文:]

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RNA技术