摘要:Chemicals needed:GlucoseL-(+)-ArabinoseSigma Cat # A3256 L-Rhamnose Sigma cat # R3875 Cb (carbenicillin) Sigma cat # 1389DL-p-ChlorophenylalanineSigma cat # C6506 Media Recipes:YEG recombination plate[阅读全文:]
摘要:近年来的研究表明，将与mRNA对应的正义RNA和反义RNA组成的双链RNA(dsRNA)导入细胞 ，可以使mRNA发生特异性的降解，导致其相应的基因沉默。这种转录后基因沉默机制(post-transcriptional gene silencing, PTGS)被称为RNA[阅读全文:]
摘要:在植物、昆虫、原生动物体内都发现了自然存在的RNAi现象，实验表明通过目的基因序列的双链RNA可以诱导产生基因沉默现象。要制备较长的dsRNA，最简单的方法就是体外转录合成双向的RNA。如果研究对象不是哺乳动物细胞，那要开[阅读全文:]
摘要:Chemicals needed:GlucoseL-(+)-ArabinoseSigma Cat # A3256 L-RhamnoseSigma cat # R3875 Cb (carbenicillin)Sigma cat # 1389DL-p-ChlorophenylalanineSigma cat # C6506Media Recipes:YEG recombination plates:1[阅读全文:]
摘要:RNA interference (RNAi ) by double stranded RNA (dsRNAs) molecules of approximately 20-25 nucleotides termed short interfering (siRNAs) is a powerful method for preventing the expression of a particul[阅读全文:]
摘要:RNA 酶污染是全体从事 RNA 相关实验的人员的头号公敌。即使是100度高温或者是高压灭菌也不能完全灭活无孔不入的 RNases 。因而相关的实验中往往要用到RNA 酶抑制剂。最常用的 RNA 酶抑制剂 Human Placental ribonucleas[阅读全文:]
摘要:RNA interference (RNAi) is a biological process in which the introduction of double-stranded RNA (dsRNA) into a cell results in targeted post-transcriptional gene silencing.Historically, RNAi has been[阅读全文:]
摘要:RNA interference (RNAi ) by double stranded RNA (dsRNAs) molecules of approximately 20-25 nucleotides termed short interfering (siRNAs) is a powerful method for preventing the expression of a particul[阅读全文:]