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"蛋白质技术" 分类下的词条该分类下有408个词条创建该分类下的词条

Crystallization of Kinesin Family Motor Proteins
词条创建者:admin创建时间:12-09 22:09
标签: Crystallization

摘要:Motor proteins of several kinesin family groups have now been crystallized: monomeric Kinesin-1 motor domains from human,rat and Neurospora (Kull et al.,1996; Sack et al.,1997; Song et al.,2001)and di[阅读全文:]

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Crystallization Trials
词条创建者:admin创建时间:12-09 22:09
标签: Crystallization Trials

摘要:Materials Milipore filter type HA 0.45 micron Culture plates (Linbro model 76-033-05)Protein in DDW or HEPES buffer (10 mg/ml)Vacuum grease Syringe with 18 G needleProcedure1.Millipore filter all solu[阅读全文:]

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Protein Syntheses in Cell Free Systems
词条创建者:admin创建时间:12-09 22:09
标签:

摘要:Materials •Suspension culture of fibroblast cells (1 liter)•35 mM Tris-HCl,pH 7.4,140 mM NaCl (TBS buffer)•10 mM Tris-HCl,pH 7.5,10 mM KCl,and 1.5 mM magnesium acetate (TBS-M)•10X [阅读全文:]

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Kamps's Western Blotting Protocol
词条创建者:admin创建时间:12-09 22:09
标签: Kamps' s

摘要:ECL or autoradiography?ECL is an appealing technique because it is quick and very sensitive and does not expose the investigator to radioactivity.The use of radioiodinated protein A to detect bound an[阅读全文:]

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METHOD for Western Blots
词条创建者:admin创建时间:12-09 22:09
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摘要:1.While your SDS-PAGE gel is running,make your transfer buffer and chill to 4℃.Check that you have a frozen buffer dam is ready (stored in freezer next to Shikha,top shelf to the right side--looks lik[阅读全文:]

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CARBONATE WESTERN TRANSFER SOLUTIONS
词条创建者:admin创建时间:12-09 22:09
标签: CARBONATE TRANSFER

摘要:Dunn,Anal.Biochem.1986: 157 1.5L 1.0L10mM NaHCO31.26gchill to 4oC0.84g3mM Na2CO30.477gpH ~9.90.318gin 20% MeOH300mL200mL1.Blot: no need to presoak gell.Transblot: 1-2H @ 1A keep T<35℃Miniblot: 1H @ 25[阅读全文:]

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Silver Staining of SDS-PAGE Gels(SDS-聚丙烯酰胺凝胶电泳银染)
词条创建者:admin创建时间:12-09 22:09
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摘要:1. Rinse gel on glass plate briefly with distilled water.2. Wash gel in fresh 50% ETOH-12% HAc, with shaking, 3 times, 1 hour each. (Fix IEF gel ON in 10% TCA).3. Wash gel four times with 10% ETOH-5% [阅读全文:]

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SDS-PAGE电泳小问答
词条创建者:admin创建时间:12-09 22:09
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摘要:Q:SDS-PAGE电泳的基本原理? A:SDS-聚丙烯酰胺凝胶电泳,是在聚丙烯酰胺凝胶系统中引进SDS(十二烷基硫酸钠),SDS能断裂分子内和分子间氢键,破坏蛋白质的二级和三级结构,强还原剂能使半胱氨酸之间的二硫键断裂,蛋白质在[阅读全文:]

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SDS-PAGE (PolyAcrylamide Gel Electrophoresis)聚丙烯酰胺凝胶电泳(图)
词条创建者:admin创建时间:12-09 22:09
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摘要:The purpose of this method is to separate proteins according to their size, and no other physical feature. In order to understand how this works, we have to understand the two halves of the name: SDS [阅读全文:]

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EMSA(PROMEGA)实验 问题与解答
词条创建者:admin创建时间:12-09 22:09
标签: EMSA(PROMEGA)

摘要:凝胶迁移实验以下问题是以Promega公司试剂盒为例。1)什么是凝胶迁移或电泳迁移率实验?凝胶迁移或电泳迁移率实验(EMSA)是一种研究DNA结合蛋白和其相关的DNA结合序列相互作用的技术,可用于定性和定量分析。这一技术最初[阅读全文:]

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蛋白质技术