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EMSA(PROMEGA)实验 问题与解答
词条创建者:admin创建时间:12-09 22:09
标签: EMSA(PROMEGA)

摘要:凝胶迁移实验以下问题是以Promega公司试剂盒为例。1)什么是凝胶迁移或电泳迁移率实验?凝胶迁移或电泳迁移率实验(EMSA)是一种研究DNA结合蛋白和其相关的DNA结合序列相互作用的技术,可用于定性和定量分析。这一技术最初[阅读全文:]

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聚丙烯酰胺凝胶电泳
词条创建者:admin创建时间:12-09 22:09
标签:

摘要:实验试剂:试剂贮备液:1、 1mol/L HCl48ml,Tris36.6g,TEMED0.23ml,加蒸馏水配成100ml,pH8.9。2、 1mol/L HCl48ml,Tris5.98g,TEMED0.46ml,加蒸馏水配成100ml,pH6.7。3、 Acr28g,Bis0.735g,加蒸馏水配成100ml。4、 Acr10g,Bis2[阅读全文:]

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Protein Electrophoresis
词条创建者:admin创建时间:12-09 22:09
标签: Protein Electrophoresis

摘要:DefinitionAmino acids, nucleotides, polypeptides, and other compounds in a colloidal state can be separated by the application of external voltages which cause charged colloid particles to move toward[阅读全文:]

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聚丙烯酰胺凝胶电泳PAGE胶的配制
词条创建者:admin创建时间:12-09 22:09
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摘要:各种浓度PAGE胶的配制(DNA电泳用)50ml体系:丙烯酰胺有效分离(bp)二甲苯青溴酚蓝丙烯酰胺30%(ml)10×TBE(ml)ddH2O(ml)TEMED(µl)过硫酸铵10%(µl)3.5% 100-10004601005.83539.1725.02505.0%100-500260[阅读全文:]

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BOILING PROTEIN PREP
词条创建者:admin创建时间:12-09 22:09
标签: BOILING PROTEIN PREP

摘要:Preparation of protein extracts for western blot 1.Grow cells to mid-log (OD600 less or equal to 1.0).2.Harvest about 5 OD's of cells in a 13X100 glass dispo tube (Use new tubes.Can be up to about 6 m[阅读全文:]

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ULTIMATE HIS-UB ASSAY FOR
词条创建者:admin创建时间:12-09 22:09
标签: ULTIMATE HIS-UB ASSAY FOR

摘要:点击查看PDF文件[阅读全文:]

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ULTIMATE HIS-UB ASSAY FOR YEAST
词条创建者:admin创建时间:12-09 22:09
标签: ULTIMATE HIS-UB

摘要:点击查看PDF文件[阅读全文:]

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Phosphoamino acid analysis:Mark Kamps's method
词条创建者:admin创建时间:12-09 22:09
标签: Phosphoamino

摘要:Recipes for the buffers are at the end of this protocol.1.Label your protein with 32Pi.Then subject the protein to SDS polyacrylamide gel electrophoresis and transfer your gel-fractionated protein to [阅读全文:]

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James Hardwick's angiotensin assay protocol
词条创建者:admin创建时间:12-09 22:09
标签: Hardwick' s

摘要:This specific procedure was developed to assay the activity of the Lck kinase expressed from a retroviral vector in rat fibroblasts.You can obviously use fewer or more cells,depending on your needs.Do[阅读全文:]

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Biosynthetic labeling
词条创建者:admin创建时间:12-09 22:08
标签: Biosynthetic

摘要:How long should cells be labeled?The ideal length of time to label cells depends on the protein of interest and the label that you are using.If you want to label an unstable protein with 35S-methionin[阅读全文:]

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蛋白质技术