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Bicinchoninic Acid (BCA) Protein Assay (Smith)
词条创建者:admin创建时间:12-09 22:08
标签: Bicinchoninic (BCA)

摘要:Considerations for useThe bicinchoninic acid (BCA)assay is available in kit form from Pierce (Rockford,Ill.).This procedure is very applicable to microtiter plate methods.The BCA is used for the same [阅读全文:]

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Measurement of Myosin Regulatory Light Chain (RLC) Phosphorylation
词条创建者:admin创建时间:12-09 22:08
标签: Measurement Phosphorylation

摘要:点击查看PDF文件[阅读全文:]

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Biuret
词条创建者:admin创建时间:12-09 22:08
标签: Biuret

摘要:Considerations for useThe principle of the biuret assay is similar to that of the Lowry,however it involves a single incubation of 20 min.There are very few interfering agents (ammonium salts being on[阅读全文:]

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Bradford protein assay
词条创建者:admin创建时间:12-09 22:08
标签: Bradford

摘要:Considerations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay.It is fairly accurate and samples that are out of range can be retested within minute[阅读全文:]

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DSP Crosslinking
词条创建者:admin创建时间:12-09 22:08
标签: DSP Crosslinking

摘要:点击查看PDF文件[阅读全文:]

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Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
词条创建者:admin创建时间:12-09 22:08
标签: Tetramethylrhodamine Succinimidyl Ester

摘要:Materials1.Drop frozen 2x cycled tubulin in a polymerization-promoting buffer.Stored at -80℃2.GTP,100 mM stock,titrate to pH ~7.Need 2 ml.3.Waterbath,set at 37℃4.SS34 rotor,5 ml and 15 ml tubes and ad[阅读全文:]

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LABELING GC-GLOBULIN WITH FLUORESCEIN
词条创建者:admin创建时间:12-09 22:08
标签: GC-GLOBULIN FLUORESCEIN

摘要:Materials1.2 mM PIPES,pH 7.0,500 ml.2.Gc-globulin (Calbiochem 345802),1 mg.3.CFSE (Carboxyfluorescein succinimidyl ester; Molecular Probes).4.Lysine,100 mM in buffer 1,10 ml.5.G25-150 desalting column[阅读全文:]

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LABELING ALPHA-ACTININ WITH IATR
词条创建者:admin创建时间:12-09 22:08
标签: ALPHA-ACTININ IATR

摘要:Materials1.Small vial and stir bar.2.Bio-Beads SM-2 in 0.7x15 cm column.3.2 mM Tris-HCl,pH 8.5,4℃,250 ml.4.Centricon-30 concentrator.5.5 mM Tris-acetate,0.1 mM DTT,pH 6.95,4℃,250 ml.6.200 mM K-borate,[阅读全文:]

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Labeling Vinculin with TRITC
词条创建者:admin创建时间:12-09 22:08
标签: Vinculin TRITC

摘要:Materials1.200 mM K-borate,pH 9.0,10 ml.2.100 mM lysine,100 mM K-borate,pH 9.0,1 ml aliquots stored at -20℃.3.2 mM Tris-HCl,pH 8.5,4℃,250 ml.4.100 mM DTT stock.5.Centricon-30 concentrator.6.5 mM Tris-[阅读全文:]

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Labeling Muscle Actin with N-(1-pyrene)iodoacetamide
词条创建者:admin创建时间:12-09 22:08
标签: Muscle N-(1-pyrene)iodoacetamide

摘要:Day 0 and 1Materials1.0.5 mM ATP,0.2 mM CaCl2,2 mM Tris-HCl,pH 8.0 at 4℃,250 ml for day 0.2.0.5 mM ATP,2 mM MgCl2,100 mM boric acid,pH 8.3 at 4℃,10 ml.3.N-(1-pyrene)iodoacetamide (Molecular probes P-2[阅读全文:]

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蛋白质技术