摘要:Day 1-2Materials1.0.5 M KCl,10 mM HEPES,pH 7.5,4℃,250 ml.2.0.5 M KCl,50 mM HEPES,pH 8.0,4℃,250 ml.3.Bio-Beads SM-2 in a 0.7x15 column.4.20 mM KCl,20 mM PIPES,pH 7.0,4℃,250 ml.5.IATR (tetramethylrhodam[阅读全文:]
摘要:Day 0 and 1Materials1.0.5 mM ATP,0.2 mM CaCl2,1 mM PIPES,pH 6.8 at 4℃,250 ml for day 0.2.0.5 mM ATP,0.2 mM CaCl2,70 mM KCl,150 mM PIPES,pH 7.0,1 ml.3.16 mM ATP,0.2 mM CaCl2,5 mM DTT,5 mM lysine,10 mM [阅读全文:]
摘要:Day 0 and 1Materials1.0.5 mM ATP,0.2 mM CaCl2,2 mM Tris-HCl,pH 8.0 at 4℃,250 ml for day 0.2.100 mM KCl,100 mM boric acid,pH 8.4 at 4℃,10 ml.3.0.5 mM ATP,2 mM MgCl2,100 mM KCl,2 mM DTT,2 mM PIPES,pH 7.[阅读全文:]
摘要:Day 0 and 1Materials1.0.5 mM ATP,0.2 mM CaCl2,2 mM Tris-HCl,pH 8.0 at 4℃,250 ml for day 0 and 1000 ml for day 1.2.100 mM KCl,100 mM boric acid,pH 8.4 at 4℃,10 ml.3.IATR (tetramethylrhodamine iodoaceta[阅读全文:]
摘要:点击查看PDF文件[阅读全文:]
摘要:This method uses carboxyfluorescein succinimidyl ester (CFSE)rather than fluorescein isothiocyanate,resulting in more reliable labeling.Succinimidyl esters are excellent reagents for amine modificatio[阅读全文:]
摘要:一、目的： 1．学习SDS—PAGE测定蛋白质分子量的基本原理。 2．掌握垂直板型电泳的基本操作技术。 二、原理： 由实验十五中已知，蛋白质在聚丙烯酰胺凝胶中电泳时，它的迁移率取决于它所带净电荷以及分子的大小和形状等因[阅读全文:]
摘要:点击查看PDF文件[阅读全文:]
摘要:点击查看PDF文件[阅读全文:]
摘要:点击查看PDF文件[阅读全文:]