生命经纬知识库>> 分类 >> 实验技术 >> 蛋白质技术

"蛋白质技术" 分类下的词条该分类下有408个词条创建该分类下的词条

Too many bands on a Western blot
词条创建者:admin创建时间:12-09 22:10
标签: Too many bands Western blot

摘要:Western- or immunoblotting is a commonly employed technique for the detection of protein antigens in complex mixtures. Samples are first separated by SDS-polyacrylamide gel electrophoresis. The separa[阅读全文:]

编辑:0次| 浏览:1263次
Tips and hints for the storage of antibodies
词条创建者:admin创建时间:12-09 22:10
标签: Tips and hints the storage antibodies

摘要:Storage of lyophilized antibodies All our products are shipped lyophilized (freeze-dried). Unlabeled antibodies are stable without loss of quality at ambient temperatures for several weeks or even a f[阅读全文:]

编辑:0次| 浏览:1501次
Western Blot试剂的准备
词条创建者:admin创建时间:12-09 22:10
标签: Western Blot试剂

摘要:1. 30%储备胶溶液:丙烯酰胺(Acr)29.0g           亚甲双丙烯酰胺(Bis)1.0g 混匀后ddH2O,37℃溶解,定容至100ml,棕色瓶存于室温。2. 4×分离胶缓冲液(1mol/L Tris-HClPH 8.8)Tris18.17g加ddH2O溶解,浓盐酸调PH 8.8,[阅读全文:]

编辑:0次| 浏览:1622次
Western Blot原理
词条创建者:admin创建时间:12-09 22:10
标签: Western Blot原理

摘要:Western免疫印迹(Western Blot)是将蛋白质转移到膜上,然后利用抗体进行检测。对已知表达蛋白,可用相应抗体作为一抗进行检测,对新基因的表达产物,可通过融合部分的抗体检测。原理与Southern或Northern杂交方法类似,但We[阅读全文:]

编辑:0次| 浏览:1858次
Far Western Protocol
词条创建者:admin创建时间:12-09 22:10
标签: Far Western Protocol

摘要:1. Run samples out on a gel. For bacterially expressed proteins, generally 5 μl is plenty (1ml cell cμlture; cells resuspeded in 50 μl loading buffer). Run the gels (BioRad mini gels) at 195 [阅读全文:]

编辑:0次| 浏览:1226次
Western Blotting Using the SemiPhor Semi-Dry Transfer Unit
词条创建者:admin创建时间:12-09 22:10
标签: Western Blotting the SemiPhor Semi-Dry Transfer Unit

摘要:1.Remove the stacking gel from the gel. Measure the gel and record its size. Do not pre-soak the gel. Do not cut a corner off the gel, as this may allow a short circuit and inefficient transfer. Blot [阅读全文:]

编辑:0次| 浏览:1851次
Far-Western Blotting
词条创建者:admin创建时间:12-09 22:10
标签: Far-Western Blotting

摘要:Studying Protein Interactions by Far-Western Blotting Far-Western blotting was originally developed to screen protein expression libraries with 32P-labeled glutathione S-transferase (GST)-fusion prote[阅读全文:]

编辑:0次| 浏览:1253次
Western Blotting with Monoclonal Ant
词条创建者:admin创建时间:12-09 22:10
标签: Western Blotting Monoclonal Ant

摘要:Sample PreparationFor Protein Concentration Determination of Cell Culture 1.Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline (PBS). 2.Aspirate exce[阅读全文:]

编辑:0次| 浏览:1282次
western blotting操作手册
词条创建者:admin创建时间:12-09 22:10
标签: western blotting 操作手册

摘要:Running Protein GelsSolutions10X Running Buffer (0.25 M Tris, 1.92 M glycine, 1% SDS)121 g Tris577 g glycine40 g SDSddh20 to 4 L (check pH at 1:10 dilution (pH=~8.8)).5X Sample Buffer (0.3125 M Tris p[阅读全文:]

编辑:0次| 浏览:1168次
Western Blotting with Alkaline Phosp
词条创建者:admin创建时间:12-09 22:10
标签: Western Blotting Alkaline Phosp

摘要:Western Blotting with Alkaline Phosphatase ConjugatesSAMPLE PREPARATIONFor Protein Concentration Determination of Cell Culture1.Decant medium from 10cm dish of adherent cells and rinse plate rapidly w[阅读全文:]

编辑:0次| 浏览:1421次
 共408条 12345678910››... 41

蛋白质技术