摘要:1. Prepare a solution of 10X annealing buffer.10 mM MgCl2 , 200 mM Tris-HCl, pH 8.0 2. Prepare an aliquot of the oligonucleotide probe in an eppendorf tube.3. Add a molar excess of oligonucleotide tar[阅读全文:]
摘要:Preparation of G+A Marker Author: Long-Cheng Li Source: Protocol Online Abstract: Simplified method for preparing G+A ladder run along with footprinting reaction. It's much simple than the o[阅读全文:]
摘要: Objective:This protocol allows rapid detection of transformation success when primers are available to allow determination of correct ligation products by size or hybridization. Procedures:Start => [阅读全文:]
摘要: DNA fingerprinting Yicheng Cao, BAC DNA preparation Miniprep with AutoGen 740 Innoculate 3-4 ml LB with 20µg/ml chloramphenicol Grow at 37 °C overnight with shaking Isolate BAC DNA with Aut[阅读全文:]
摘要:当前，SNPs研究在国际上有很大的进展，NATURE、Science、New England 、PNAS等顶尖杂志在近几年发表了大量的论文，国内各种医学权威期刊几乎每期都刊登关于SNPs的论文。当然，国内与国外相比还是有很大差距的，这体现在国内的[阅读全文:]
摘要:一、 材料基因组 DNA (大于50kb,分别来自不同的材料)。二、设备电泳仪及电泳槽， 照相用塑料盆5只，玻璃或塑料板(比胶块略大) 4块，吸水纸若干，尼龙膜(依胶大小而定)，滤纸 ，eppendorf管(0.5ml)若干。三、试剂1、限制性内切酶([阅读全文:]
摘要:Description: For each SNP two PCR primers and a forward and reverse single base extention SNP primer design was attempted. After obtainign uniquely mapped SNPs with other known SNPs marked in the sequ[阅读全文:]
摘要: DNase I Footprinting assay is a method of studying DNA -protein interaction and identifying the DNA-sequence to which a protein binds. First, a target DNA-fragment about 100-300 bp in length is eithe[阅读全文:]
摘要: Analysis of the interaction of proteins with either DNA or RNA sequences by in vivo footprinting involves two steps: (i) the in situ modification of nucleic acids by the footprinting reagent and (ii[阅读全文:]
摘要: (1) Core out a single, well separated plaque from the agar plate using a 200µl pipette tip and place it into 500µl of SM buffer in a 1.5ml microtube:SM buffer5.8g of NaCl 2.0g of MgSO4.7H[阅读全文:]