问：我想知道合成肽与KLH和BSA偶联的详细步骤，谢谢了！

答：Basically, it depends on the feature of your synthesized peptide. Especially, if some cyestine group on your peptide, it is okay to conjuate your peptide with KLH or BSA via sulfo-SMCC. If you dont care about some a.a contain free-COOH will be disrupted, then you can select EDC as your linker to get your peptide and BSA together.

One advice, do not use HCO-(CH2)5-CHO as linker, it will cause mutant and subsequently cause cancer.

More information, you can see some protocols from some reagent suppliers, as for our lab, we choose PIERCE's kits, very easy to do such experiments with its pre-activated kits.