Description Recent advances in the imaging technique electron microscopy (EM)have improved the method,making it more reliable and rewarding,particularly in its description of three-dimensional detail。This book will help biologists from many disciplines understand modern EM and the value it might bring to their own work。The book?s five sections deal with all major issues in EM of cells: specimen preparation,imaging in 3-D,imaging and understanding frozen-hydrated samples,labeling macromolecules,and analyzing EM data。Each chapter was written by scientists who are among the best in their field,and some chapters provide multiple points of view on the issues they discuss。Each section of the book is preceded by an introduction,which should help newcomers understand the subject。The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules,helping us toward the goal of an atomic resolution understanding of living systems. 全书分4部分 1.Preparing Cells for Electron Microscopy Introduction: McIntosh - A new era in electron microscopy of cells Preparative Methods for Cellular Electron Microscopy 1.The physics of rapid cooling and its implications for cryo-immobilization of cells Imaging Fixed Cells in Three Dimensions 5.Understanding microtubule organizing centers by comparing mutant and wild-type structures with electron tomography Imaging Frozen-Hydrated Cells and Cell Parts 14.Electron tomography of bacterial chemotaxis receptor assemblies Localizing Macromolecules in Cells 19.3-D Immuno-localization with plastic sections Aspects of Data Collection and Analysis Instrumentation for image acquisition 26.Application of Energy Filtered Electron Microscopy to Tomography of Thick,Selectively Stained Biological Samples Computational methods for the analysis of cellular data 29.Structure determination in situ by averaging of tomograms |
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