用DAPI进行细胞染色 DAPI的配置 Stock solution of 1mg/ml in ddH2O; working solution of 0.25mg/ml to 50mg/ml in ddH2O or PGM. 1.Place sample on slide. 2.Add a few drops working solution. 3.Squash and view. Staining ? DAPI Staining for Cells: Separately DAPI staining: • Wash the cells twice with PBS • Incubate with 1: 750 of stock DAPI for 5 min in dark • Wash 2 x 5 min with PBS in dark • Rinse with water for a few seconds • Air dry at RT in dark • Mount with antifade solution (Vectashield, Co#: H-1000, Vector Lab) 5 µl for each d12 mm coverslip, 25 µl for square coverslip and 50µl for full size coverslip • Seal the coverslip with nail polish • Store in 4 C up to 2 weeks DAPI stock • 4’, 6-Diamidino-2-phenylindole dihydrochloride hydrate (DAPI) from Sigma (Cat# D-9542), 1 mg 1mg/0.5 ml in H2O and stocked at ?20 in the dark Staining with antifade mounting solution: • Wash the cells twice with PBS • Mount with antifade solution with DAPI (Vectashield, Co#: H-1200,Vector Lab, 1.5 µg/ml) at 1:3 dilution with Vectashield H-1000 Volume used as above • Store in 4 C up to 2 weeks |
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