1.Yeast (93.75 g) + Super Q Water (562.5 ml) per flask. 2.Place in incubator at 37°C for 20 minutes. 3.Heat to 90-100°C for 5 minutes. 4.Cool on ice. 5.Pour into centrifuge bottles and centrifuge at 2500 rpms for 30 minutes (4°C). 6.Filter supernatant through glass pads twice. 7.Measure into bottles and label. 8.Autoclave for 2-5 minutes at 115°C. 9.Freeze in 45 and 90 ml aliquots. 125 g yeast per 750 ml water = 1 batch Yield is approximately 700 ml. |
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