Composition per liter: NaCl ……19.45g Agar ……15.0g MgCl2……8.8g Peptone ……5.0g Na2SO3……3.24g CaCl2……1.8g Yeast extract……1.0g KCl……0.55g NaHCO3……0.16g Ferric citrate……0.1g KBr ……0.08g SrCl2……0.03g H3BO3 ……0.02g Na2HPO4……8.0mg Na2SiO3……4.0mg NaF ……2.4mg NH4NO3 ……1.6mg Biphenyl……1.0mg pH 7.6 ± 0.2 at 25°C Preparation of Medium: Add components, except biphenyl, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure –121°C. Pour into sterile Petri dishes or leave in tubes. After agar solidifies, aseptically add a few crystals of biphenyl to each plate. Use: For the cultivation and maintenance of biphenyl-utilizing marine bacteria, such as Cycloclasticus pugetii. |
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