取粪便，进行倍比稀释，涂布或滴种于乳酸杆菌选择培养基上，如LC或MRS，进行需氧培养或厌氧培养，厌氧培养结果会好一些，48h或72h即可观察结果，并通过革兰氏染色观察形态，G(+)杆菌，无芽胞，球杆状或杆状，排成栅栏或链状，无动力，为兼性厌氧菌。

可参考方法：

1、点击下面的链接下载

猪肠道中抑制大肠杆菌的乳酸菌的分离与筛选.pdf

2、methods:Isolation of lactic acid bacteria. LAB were isolated from intestinal contents of 10 months old Wistar rat. The rat was fed with the Nutrilab-rodent feed procured from Tetragon Chemie Pvt Ltd, Bangalore, India. One gram of intestinal content was suspended in 9 ml of 0.85 % sterile saline. The solution was further diluted with saline and 0.1 ml of the solution was spread on MRS agar and incubated in anaerobic jar at 37 ℃ for 2 days. The colonies were randomly picked and transferred to MRS broth. Further purification was achieved through streaking on MRS agar to pick up pure colonies. The cultures were further stored in MRS agar overlaid with 50 % glycerol at minus 20 ℃. A total of twenty-three isolates were tested for their ability to produce bacteriocins.

Reference:ISOLATION OF BACTERIOCINOGENIC LACTIC ACID BACTERIA FROM RAT INTESTINE.pdf