Composition per liter:

Agar ……10.0g

Malt extract ……0.1g

Yeast extract……0.1g

Artificial seawater…… 1.0L

Artificial Seawater:

Composition per liter:

NaCl ……27.5g

MgSO₄·7H₂O……6.78g

MgCl₂·6H₂O ……5.38g

KCl……0.72g

NaHCO₃…… 0.2g

CaCl₂·2H₂O……1.4g

Preparation of Artificial Seawater: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to artificial seawater and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Cochliopodium clarum, Heteramoeba clara, Ling***oeba leei, Paramoeba pemaquidensis, and Vannella species.