Running Protein Gels Solutions 10X Running Buffer (0.25 M Tris, 1.92 M glycine, 1% SDS) 121 g Tris 577 g glycine 40 g SDS ddh20 to 4 L (check pH at 1:10 dilution (pH=~8.8)). 5X Sample Buffer (0.3125 M Tris pH 6.8, 10% SDS, 50% glycerol, 0.005% bromophenol blue, 25% 2-mercaptoethanol) 1.56 ml 2M Tris pH 6.8 1 g SDS 5 ml glycerol 0.2 ml 0.25% solution of bromophenol blue 2.5 ml 2-mercaptoethanol ddH20 to 10ml. 30:0.8 Acrylamide:bis 0.5 M Tris pH 6.8 10% SDS 10% APS TEMED 3 M Tris pH 8.8 |
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