生命经纬知识库 >>所属分类 >> 蛋白质技术   

Tips and hints for the storage of antibodies

标签: Tips and hints the storage antibodies

顶[0] 发表评论(445) 编辑词条

Storage of lyophilized antibodies

All our products are shipped lyophilized (freeze-dried). Unlabeled antibodies are stable without loss of quality at ambient temperatures for several weeks or even a few months. They can be stored at 4 ℃ for several years.Fluorescence-labeled antibodies should be reconstituted immediately upon delivery.

After reconstitution by addition of water storage conditions depend on the type of antibody:

Monoclonal antibodies

Ascites. When ascites is reconstituted, we usually add small amounts of azide or thimerosal to prevent microbial growth. Ascites should be stored frozen. Monoclonals usually do not suffer from repetitive freeze-thawing but you may aliquot them into small samples to avoid too many freeze-thaw cycles. Prolonged storage at 4 ℃ is not recommended! Unlike serum, ascites may contain proteases that will ultimately degrade the antibodies. Addition of protease inhibitors helps to slow degradation.

Hybridoma supernatant should stored as ascites.

Purified IgG. Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/ml protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Rabbit antisera

Antisera are more robust than monoclonals. With anti-microbials added, they may be stored at  ℃. Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage, however, is preferable.

Polyclonal affinity purified antibodies

Affinity purified antibodies are less robust than sera, since protease inhibitors are also removed during purification. Hence, storage at 4 ℃ for prolonged periods (i.e. several weeks), is not recommended.

附件列表


→如果您认为本词条还有待完善,请 编辑词条

上一篇蛋白质三级机构预测方法的分析与评价 下一篇western-blot实验方法

词条内容仅供参考,如果您需要解决具体问题
(尤其在法律、医学等领域),建议您咨询相关领域专业人士。
0

收藏到:  

词条信息

admin
admin
超级管理员
词条创建者 发短消息   

相关词条