生命经纬知识库 >>所属分类 >> PCR技术   

Footprinting

标签: 暂无标签

顶[0] 发表评论(10) 编辑词条

 
 
 
DNase I Footprinting assay is a method of studying DNA -protein interaction and identifying the DNA-sequence to which a protein binds. First, a target DNA-fragment about 100-300 bp in length is either PCR generated or cut from a vector and then uniquely labeled (at only one end) and incubated with protein (usually nuclear extract), followed by controlled digestion with DNase I which cut the probe randomly but only once. The digested DNA is recovered from the reaction and resolved on a polyacrylamide gel along with G+A chemical sequencing reaction which uses the same probe as template. The regions bound by proteins will be protected from DNase I digestion and will be shown as a blank area on the gel track, while the exact protein-bound sequence can be read out by comparing the location of the blank with the sequencing reaction.

附件列表


→如果您认为本词条还有待完善,请 编辑词条

上一篇Single Primer ("Semi-Random") PCR 下一篇定量PCR技术

词条内容仅供参考,如果您需要解决具体问题
(尤其在法律、医学等领域),建议您咨询相关领域专业人士。
0

收藏到:  

词条信息

admin
admin
超级管理员
词条创建者 发短消息   
  • 浏览次数: 962 次
  • 编辑次数: 1次 历史版本
  • 更新时间: 2009-12-09

相关词条