Prior to Extractions ? Bake all necessary glassware, metal spatulas, mortars, and pestles overnight in a 200℃ oven after wrapping them in aluminum foil. For each sample, you should minimally prepare one mortar and pestle, one homogenizer and one spatula. ? DEPC-treat a sufficient volume of water for all dilutions (500 mL is usually plenty). ? Prepare all reagents and solutions.
Day 1
Place all mortars to be used in liquid nitrogen to cool. Heat homogenizers to 65℃ and acquire dry ice. After the mortars are cooled, nestle them in the dry ice.