The Easiest Route to Guaranteed Silencing Increasing numbers of researchers are using small interfering RNAs (siRNAs) to reduce the expression of specific mammalian genes. Applications of siRNA induced RNA interference (RNAi ) include gene function analysis, pathway elucidation, functional genomics, and drug target validation, to name just a few. The Importance of Optimized siRNA Design One of the key components of success in any siRNA experiment is siRNA design. Well-designed siRNAs efficiently target the mRNA of interest and induce minimal off-target effects. Poorly designed siRNAs, in contrast, lead to little or no gene silencing, or they reduce the expression of nontargeted genes. Unfortunately, designing an effective siRNA can be a time consuming and labor intensive process (Figure 1).
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