Hybridization for Spectral Karyotyping (SKY)

Reagents

dH ₂O
      Ethanol, absolute (70%, 90%, and 100%) 
      Formamide, deionized 
      Ambion, Cat. No. 9342 
      HCL, 1N 
      Rubber Cement 
      SSC, 20X

Preparation

70% Formamide/2X SSC 
      20X SSC 10 ml 
      dH ₂O 20 ml 
      deionized formamide 70 ml 
      Adjust to pH 7.0 with 1N HCL 
      Aliquot and store at –20°C. 
      Pre-cool 70% ethanol to 0°C

详细操作步骤请点击下面的链接下载：

Hybridization for Spectral Karyotyping (SKY) .pdf