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amplifiable dna from bones-Molecular Biology

标签: amplifiable bones-Molecular

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does anyone have a reasonably successful protocol for isolating dna from bones---bones that are highly compromised in integrity---i.e.,really bad shape?  I've tried and am well seasoned in the traditional methods,e.g.sds pro K,salting out,PIC,silica/chaotropic ,qiagen,zymo,ect.Sometimes these things work and sometimes they don't.Surely there is a protocol that works sucessfully most of the time.Thank you

-Bo Scales-

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Bo,

Actually,the company I work for (Gentra)did some DNA purifications from ancient bone samples using the PUREGENE kit.If you want,I can send you the paper on this (I think that it is still in press)for you to look over.Please email me at if you are interested and I can send it to you.

AronD

-AronD-

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get yourself this months Euro edition of Biotechniques (a free really nice journal),you can also get  it online for free as well (do  a google search for biotechniques).In there they have an article on DNA extraction from bones from musem specimens.Ideal for what you want i reckon.

-sbidw-

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opps forgot the citation:

BioTechniques 30:732-736 (2001)

Good luck.

-sbidw-

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sbidw had a great idea there!  You can also find it in the U.S.version of BioTechniques.That citation is 22:402 (1997).

-AronD-

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