Materials: phenol:chloroform (1:1) chloroform 1.Add an equal volume of buffer-saturated phenol:chloroform (1:1) to the DNA solution. 2.Mix well. Most DNA solutions can be vortexed for 10 sec except for high molecular weight DNA which should be gently rocked. (If using Phase-Lock Gel, follow procedure M.1) 3.Spin in a microfuge for 3 min. 4.Carefully remove the aqueous layer to a new tube, being careful to avoid the interface. 5.(Steps 1-4 can be repeated until an interface is no longer visible) . 6.To remove traces of phenol, add an equal volume of chloroform to the aqueous layer. 7.Spin in a microfuge for 3 min. 8.Remove aqueous layer to new tube. 9.Ethanol precipitate the DNA. |
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