Genotyping Transgenic Rodents by PCR

This is how we test mice and rats for the presence of the transgene by PCR. It is provided for those investigators who are unfamiliar with DNA genotyping and who need to establish a genotyping method.

1. Quantitate tail DNA

2. Prepare 20 µl aliquots of tail DNA diluted to 200 ng/microliter in autoclaved water.

3. Heat DNA for 1 minute in a dry bath set for 95 degrees C.

spin down tubes

place on ice

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