Quick question as I have read all sorts of views on this before: How long can bisufite-treated DNA be stored in -80℃ freezer before it degrades. Is there any reason why it should degrade quicker than untreated DNA?? Jon -spjgjsm- -------------------------------------------------------------------------------- Jon, I resuspend my bisulfite treated DNA in fresh autoclaved TE buffer and store at -20C. I have stored such DNA upto 6 months with no detectable loss of DNA and by then I have used up my batch and I have to make another batch. Hope this helps. Cheers Nick -methylnick- -------------------------------------------------------------------------------- I agree with Nick. No problem for six months although some suggest no more than one month. -pcrman- -------------------------------------------------------------------------------- cheers! jon -spjgjsm- -------------------------------------------------------------------------------- Make sure you store in TE, rather than water. Bis treated DNA stored in water goes off quite fast. -Chris Harris- -------------------------------------------------------------------------------- |
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