This technique appeared as an article in Biotechniques, Vol.14, No.3, pp412-414. This article is a report by Kristian Gundersen, Theresa Hanley and John Merlie, on the use of micropipette bevelling to increase transgenic (pronuclear injection) embryo yield. A slurry of grit 120 silicon carbide is used in a beaker on a stir plate to provide a gentle yet abrasive force. Micropipettes drawn out previously on a vertical or horizontal puller can be further modified by the immersion of their tips into the rotating slurry at approximately 45 degrees for 15-30 seconds. This imparts an aperture which is less prone to clogging and also reduces the shearing force applies to the DNA as it is expelled through the glass needle. These factors together serve to increase the viability of injected eggs and the subsequent successful transgene integration. The vendor for the silicon carbide grit is as follows: Buehler Ltd 41 Waukegan Rd. Lake Bluff, IL 60044 Toll-free Number: N/A Phone Number: 708 295-6500 Fax Number: 708 295-7979 There is a minimum order of $50. |
→如果您认为本词条还有待完善,请 编辑词条
上一篇Microinjection DNA Purification 下一篇TSA Treatment of Mammalian Cells (PROT09)
词条内容仅供参考,如果您需要解决具体问题
(尤其在法律、医学等领域),建议您咨询相关领域专业人士。
0