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搜索“TAG:Alkaline”找到相关内容2篇,用时1.035124秒

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Alkaline Southern Blotting Procedure
词条创建者:admin创建时间:12-09 23:16
标签: Alkaline Southern Blotting Procedure

摘要:Author: Suzanne GerttulaDate: March 14,1994 1.Digest between 1 and 10 μg Genomic DNA. I digest at 37 C for about six hours over the course of a day. Spin down briefly, heat to 65 ℃ for 10 min and t[阅读全文]

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Alkaline Lysis Plasmid Prep(碱裂解法制备质粒DNA)
词条创建者:admin创建时间:12-09 23:14
标签: 碱裂解法 制备质粒

摘要:Alkaline lysis miniprep1. Grow bacteria overnight in 37℃shaking incubator, with lids very loose and taped on. I normally use 5 ml of liquid medium in a 50ml conical bottom tube. Be sure to include the[阅读全文]

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