摘要:Author: Suzanne GerttulaDate: March 14,1994 1.Digest between 1 and 10 μg Genomic DNA. I digest at 37 C for about six hours over the course of a day. Spin down briefly, heat to 65 ℃ for 10 min and t[阅读全文]

摘要:Alkaline lysis miniprep1. Grow bacteria overnight in 37℃shaking incubator, with lids very loose and taped on. I normally use 5 ml of liquid medium in a 50ml conical bottom tube. Be sure to include the[阅读全文]