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搜索“TAG:DNAse”找到相关内容6篇,用时1.057382秒

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DNase?I?Footprinting
词条创建者:admin创建时间:12-09 23:24
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摘要: An end labeled DNA probe is incubated with a purified DNA-binding factor or with a protein extract. The unprotected DNA is then digested with DNase I such that on average, every DNA molecule is cut o[阅读全文]

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DnaseI Footprinting
词条创建者:admin创建时间:12-09 23:24
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摘要:Protocol:Combine an appropriate concentration of probe that has been labeled on one strand (100-150 fmol probe has worked so far) with the appropriate concentration of protein in a total volume of 50&[阅读全文]

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DNaseI?Footprintint
词条创建者:admin创建时间:12-09 23:24
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摘要:DNaseI FootprintintSolutions10X Binding Buffer200 mM Tris 8.0 200 m l 1M Tris pH 8.0500 mM NaCl 100 m l 5M NaCl10 mM EDTA 20 m l 0.5 M EDTA pH 8.0680 m l Qstore at room temperatureDNaseI Dilution Buff[阅读全文]

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DnaseI Footprinting
词条创建者:admin创建时间:12-09 23:11
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摘要:Sugden lab,McArdle Laboratory for Cancer Research, University of Wisconsin-Madison Medical School DnaseI Footprinting(Based upon the protocol from the Promega Core Footprinting Kit)5x Binding Buffer ([阅读全文]

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DNase I Treatment Of Total RNA
词条创建者:admin创建时间:12-09 22:48
标签: DNase I Treatment Total RNA

摘要:Reagents1 U/ul DNase I from Epicentre Technologies10X DNase I buffer (200 mM Tris, pH 8.4, 20 mM MgCl2, 500 mM KCl)For 1ml:200 mM Tris, pH 8.4200 ul 1M Tris, pH 8.4500 ul 1M KCl20 ul 1 M MgCl2280 ul D[阅读全文]

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DNAse Treatment Of Total RNA
词条创建者:admin创建时间:12-09 22:48
标签: DNAse Treatment Total RNA

摘要:OverviewMost RNA extractions procedures yield RNA, with minimal or no contamination with DNA, that is suitable for applications such as northern blot, constructions of cDNA libraries or microarray hyb[阅读全文]

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