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Fisheer氏综合征
词条创建者:admin创建时间:06-20 11:47
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摘要:Fisheer氏综合征又称,眼外肌麻痹—共济失调—深反射消失综合征;急性播散性脑脊髓神经根综合征。 【病因和机理】 病因不十分明确,目前认为有病毒感染和变态反应两种说法。也可能是异型或非典型的Guil[阅读全文]

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Foster–Kennedy氏综合征
词条创建者:admin创建时间:06-20 11:46
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摘要:Foster–Kennedy氏综合征又称额叶基底部综合征。 【病因和机理】 额叶底部肿瘤或蝶骨嵴、嗅沟脑膜瘤压迫一侧视神经,使视神经周围的蛛网膜下腔闭塞,引起视神经原发性萎缩而不出现视乳头水肿,但由于[阅读全文]

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Foville氏综合征
词条创建者:admin创建时间:06-20 11:46
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摘要:Foville氏综合征又称桥脑旁正中征群。 【病因和机理】 桥脑肿瘤、炎症及血管性病变等致面神经核或核下纤维、外展神经核、三叉神经根丝或三叉神经脊髓束核的损害。 【临床表现】 表现为病侧面神经麻痹和向[阅读全文]

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Foix氏综合征
词条创建者:admin创建时间:06-20 11:46
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摘要:Foix氏综合征又称海绵窦综合征;垂体蝶骨综合征;海绵窦血栓形成综合征。 【病因和机理】 由海绵窦外侧壁肿瘤、垂体瘤、蝶骨肿瘤及海绵窦血栓性静脉炎、海绵窦内动脉瘤等致Ⅲ、Ⅳ、Ⅵ、Ⅴ(1、2支)颅神经麻[阅读全文]

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Foix–Alajouanine氏综合征
词条创建者:admin创建时间:06-20 11:46
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摘要:Foix–Alajouanine氏综合征又称,亚急性坏死性脊髓炎综合征。 【病因和机理】 是由于脊髓血管异常并发脊髓坏死。血管异常。以静脉为主,呈蛇行状屈曲,血管壁肥厚内腔狭窄,引起血液循环障碍导致脊[阅读全文]

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FINGERPRINTING?PROTOCOL?(AGAROSE?GEL)
词条创建者:admin创建时间:12-09 23:24
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摘要:FINGERPRINTING PROTOCOL (AGAROSE GEL) * Restriction digests consist of:15.75 ml ddH2 O 1 µl 10 X buffer B (Boehringer Mannheim) 0.25 µl HindIII (40 U/µl) 3 µl DNA Set up digest[阅读全文]

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Footprinting
词条创建者:admin创建时间:12-09 23:24
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摘要:FootprintingFootprinting is a method for determining the exact DNA sequence to which a particular DNA-binding protein binds. Examples: hormone-receptor complexes that bind to their hormone response el[阅读全文]

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Fill-in?Labeling?of?DNA?Fragments
词条创建者:admin创建时间:12-09 23:24
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摘要:This protocol was designed to generate directionally end-labeled probes for DNaseI footprinting but it can be used for any application that requires end-labeled DNA probes.Solutions10 mM dNTP StocksTh[阅读全文]

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Filter binding assay简介、原理和方法
词条创建者:admin创建时间:12-09 23:22
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摘要:简介 优点:操作简单方便 缺陷:不能像EMSA或footprinting一样区分不同类型复合物 原理 双链 DNA 能够通过硝化纤维膜 (NC),但是 DNA-protein 复合物不能通过NC filter. 通过定量分析留在滤膜上的复合物,可以判断二者的[阅读全文]

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free shotgun sequencing library from miniprep BAC DNA
词条创建者:admin创建时间:12-09 23:21
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摘要:Miniprep of BAC DNA1.Inoculate 4 - 8 tubes of 3ml/tube LB with 15μg/ml chloramphenicol with BAC clone and grow oveernight. Miniprep with AutoGen 740 according to the protocol described in our Web p[阅读全文]

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FOOTPRINTING WITH DNASE1
词条创建者:admin创建时间:12-09 23:21
标签: FOOTPRINTING DNASE1

摘要:1)probe: same as used for gelshift), isolated by isotacelectrophoresis w/out EtOH ppt which can enature dsDNA 2)probe mix/rxn: volumes x # samples 1μl probe (0.1?0.5ng or 10?20kcpm)   0.15μl 20m[阅读全文]

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Fill-in Labeling of DNA : directional end label
词条创建者:admin创建时间:12-09 23:20
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摘要:http://axon.med.harvard.edu/~cepko/protocol/mike/F1.html This protocol was designed to generate directionally end-labeled probes for DNaseI footprinting but it can be used for any application that req[阅读全文]

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Freeze Squeeze PCR Product Purification from Agorose Gel
词条创建者:admin创建时间:12-09 23:15
标签: Purification PCR

摘要:Abstract: The “Freeze squeeze” method is a cost effective and technically simple procedure to isolate and prepare PCR products for DNA sequencing. Reagents•70% ethanol •3 M sodiu[阅读全文]

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Filter Binding Assay for EBNA-1
词条创建者:admin创建时间:12-09 23:14
标签: Binding Assay EBNA-1

摘要:Sugden lab,McArdle Laboratory for Cancer Research, University of Wisconsin-Madison Medical School http://mcardle.oncology.wisc.edu/sugden/Protocols/html files/Filter Binding Assay.htm Last Modified 5.[阅读全文]

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FOOTPRINTING WITH DNASE1
词条创建者:admin创建时间:12-09 23:13
标签: FOOTPRINTING DNASE1

摘要:John Mundy ,Institute of Molecular Biology,Copenhagen,Denmark http://www.arabidopsis.org/info/Protocols_Mundy2.jsp#Footprint 1)probe: same as used for gelshift),isolated by isotacelectrophoresis w/out[阅读全文]

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Frozen competent E. coli cells 【Carnegie Institution of Washington】
词条创建者:admin创建时间:12-09 23:11
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摘要:Inoculate a 5ml overnight of E.coli in LB+20 mM MgSO4.Next morning,inoculate 250 ml LB+20 mM Mg++ in a 2L flask with about 2ml overnight culture.Grow at room temp (23℃)with good aeration (250rpm)to an[阅读全文]

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Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别提取细胞核和细胞质RNA
词条创建者:admin创建时间:12-09 22:50
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摘要:T℃OS-M6 cells grow in 6-well plates, 2ml media total T℃V1PD cells grow in 100mm plates.1: Aspirate off the media from the cells and was the cell monolayers with 2x5ml (To 100mm dish) or 2x1ml (to 6-we[阅读全文]

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Footprinting
词条创建者:admin创建时间:12-09 22:29
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摘要: DNase I Footprinting assay is a method of studying DNA -protein interaction and identifying the DNA-sequence to which a protein binds. First, a target DNA-fragment about 100-300 bp in length is eithe[阅读全文]

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Far-Western Blotting
词条创建者:admin创建时间:12-09 22:10
标签: Far-Western Blotting

摘要:Studying Protein Interactions by Far-Western Blotting Far-Western blotting was originally developed to screen protein expression libraries with 32P-labeled glutathione S-transferase (GST)-fusion prote[阅读全文]

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Far Western Protocol
词条创建者:admin创建时间:12-09 22:10
标签: Far Western Protocol

摘要:1. Run samples out on a gel. For bacterially expressed proteins, generally 5 μl is plenty (1ml cell cμlture; cells resuspeded in 50 μl loading buffer). Run the gels (BioRad mini gels) at 195 [阅读全文]

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